Thermal transition of native tobacco mosaic virus and RNA-free viral proteins into spherical nanoparticles
Thermal transition of native tobacco mosaic virus and RNA-free viral proteins into spherical nanoparticles
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DOI：
10.1099/vir.0.024356-0
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发表时间：
2011-02-01
影响因子：
3.8
通讯作者：
Karpova, Olga
中科院分区：
文献类型：
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作者：
Atabekov, Joseph;Nikitin, Nikolai;Karpova, Olga
以天然烟草花叶病毒(TMV)为原料，在94℃下两步热重塑得到球形纳米粒子(SNPs)。90℃下TMV产生形状不规则、大小不等的颗粒，在94℃加热可转化为SNPs，被认为是SNPs的中间前体。除了由单个TMV病毒粒子产生的SNP单体(直径53 nm)外，还组装了大的SNP(直径100-800 nm)。SNPs的大小取决于TMV的浓度。这些SNP可以由不同形式的无RNA TMV外壳蛋白(CP)聚合体和单独的CP亚基产生。在这些情况下，似乎发生了一步SNP组装。这些结果表明，SNPs代表了一种新型的颗粒纳米平台，用于制备表面结合了外源蛋白质分子的SNPs的组合物。
Spherical nanoparticles (SNPs) were generated by two-step thermal remodelling of native tobacco mosaic virus (TMV) at 94 degrees C. Particles of irregular shape and varying size were generated by TMV at 90 degrees C. They could be converted into SNPs by heating at 94 degrees C and were considered to be intermediate precursors of SNPs. In addition to SNP monomers (53 nm diameter), generated by individual TMV virions, large SNPs (100-800 nm diameter) were assembled. The size of the SNPs depended on the TMV concentration. The SNPs could be generated by distinct forms of RNA-free TMV coat protein (CP) aggregates and individual CP subunits. A one-step SNP assembly appeared to occur in these cases. These results show that SNPs represent a new type of particle nanoplatform for producing compositions of SNPs with foreign protein molecules bound to their surface.