Ferulic acid protects against radiation-induced testicular damage in male rats: impact on SIRT1 and PARP1
Ferulic acid protects against radiation-induced testicular damage in male rats: impact on SIRT1 and PARP1
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DOI：
10.1007/s11356-017-0873-6
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发表时间：
2018-03-01
影响因子：
5.8
通讯作者：
Nada, Ahmed S.
中科院分区：
文献类型：
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作者：
EL-Mesallamy, Hala O.;Gawish, Rania A.;Nada, Ahmed S.
电离辐射破坏精子的发生，是男性不育的主要原因。因此，显然需要一种有效的辐射防护剂。本研究的目的是探讨阿魏酸（FA）对放射性睾丸损伤的潜在防护作用。成年雄性Sprague-Dawley大鼠分别接受单剂量γ辐射（5 Gy）和/或FA (50 mg/kg)，连续照射前7天。照射后7天，检测小鼠体内SIRT1 （SIRT1）、聚（adp -核糖）聚合酶1 （PARP1）、胞质钙含量、雄性生殖功能（精子头异常）及氧化应激标志物。FA显著维持活跃的精子发生。此外，它还逆转了辐照的氧化应激效应。照射组PARP1的表达和活性以及胞质钙浓度均显著升高，而SIRT1的表达和活性明显降低；相反，FA治疗阻止了这些改变。睾丸组织病理检查结果与生化分析结果一致。我们的数据首次表明，FA对辐射引起的睾丸损伤具有辐射防护作用。它通过增加睾丸SIRT1和睾酮水平以及降低氧化应激、PARP1和细胞质钙来改善精子发生。
Ionizing radiation is a major contributor to male infertility by destroying spermatogenesis. Therefore, the need for an effective radio-protective agent is evident. The objective of the present study was to investigate the potential radio-protective effect of ferulic acid (FA) on radiation-induced testicular damage. Mature male Sprague-Dawley rats were either exposed to a single-dose gamma radiation (5 Gy) and/or treated with FA (50 mg/kg), daily for 7 days before irradiation. Sirtuin1 (SIRT1), poly (ADP-ribose) polymerase 1 (PARP1), cytosolic calcium content, and the male reproductive functions (sperm head abnormality) as well as oxidative stress markers were assessed 7 days after irradiation. FA significantly maintained active spermatogenesis. Moreover, it reversed the oxidative stress effects of irradiation. The irradiated group showed marked elevation in both PARP1 expression and activity as well as in cytosolic calcium concentration, whereas SIRT1 activity and expression markedly decreased; in contrast, FA treatment prevented these alterations. Results of histopathological examination of testicular tissues indicated coincidence with those recorded by biochemical analyses. Our data show for the first time that FA had radio-protective effect against radiation-induced testicular damage. It improved spermatogenesis through increasing testicular SIRT1 and testosterone levels and decreasing oxidative stress, PARP1, and cytosolic calcium.