The dendritic cell populations of mouse lymph nodes
The dendritic cell populations of mouse lymph nodes
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DOI：
10.4049/jimmunol.167.2.741
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发表时间：
2001-07-15
影响因子：
4.4
通讯作者：
Shortman, K
中科院分区：
文献类型：
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作者：
Henri, S;Vremec, D;Shortman, K
从小鼠淋巴结(LN)分离树突状细胞(DC)，分析其表面标志，并与脾的DC进行比较。低至中等染色的LN DC的CD4和CD8的低染色被证明是由于从T细胞拾取这些标记物。排除这个伪影，可以描绘出五个LN DC子集。它们包括在脾中发现的三个群体(CD4(+)8(-)DEC-205(-)，CD4(-)8(-)DEC-205(-)，CD4(-)8(+)DEC-205(+))，尽管表达CD4的DC的发生率很低。LN DC包括两个额外的群体，其特征是相对较低的CD8表达但DEC-205的中等或高表达。两者都出现在从皮肤向LN迁移的DC中，但仅有一例局限于皮肤引流的LN，并被鉴定为表皮郎格汉斯细胞(LC)的成熟型。LC来源的DC具有以下特性：体积大；高水平的II类MHC，即使在CIITA基因缺失的小鼠中也能在一定程度上持续存在；表达非常高水平的DEC-205和CD40；表达许多髓系表面标志；不表达CD4，仅低至中等表达CD8。在皮肤移行者和LN中，假定来源于LC的DC也显示出强烈的langerin细胞内染色。
The dendritic cells (DC) of mouse lymph nodes (LN) were isolated, analyzed for surface markers, and compared with those of spleen. Low to moderate staining of LN DC for CD4 and low staining for CD8 was shown to be attributable to pickup of these markers from T cells. Excluding this artifact, five LN DC subsets could be delineated. They included the three populations found in spleen (CD4(+)8(-)DEC-205(-), CD4(-)8(-)DEC-205(-), CD4(-)8(+)DEC-205(+)), although the CD4-expressing DC were of low incidence. LN DC included two additional populations, characterized by relatively low expression of CD8 but moderate or high expression of DEC-205. Both appeared among the DC migrating out of skin into LN, but only one was restricted to skin-draining LN and was identified as the mature form of epidermal Langerhans cells (LC). The putative LC-derived DC displayed the following properties: large size; high levels of class II MHC, which persisted to some extent even in CIITA null mice; expression of very high levels of DEC-205 and of CD40; expression of many myeloid surface markers; and no expression of CD4 and only low to moderate expression of CD8. The putative LC-derived DC among skin emigrants and in LN also showed strong intracellular staining of langerin.