Expression of the PmSUC1 sucrose carrier gene from Plantago major L is induced during seed development
Expression of the PmSUC1 sucrose carrier gene from Plantago major L is induced during seed development
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DOI：
10.1046/j.1365-313x.1996.09010093.x
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发表时间：
1996-01-01
期刊：
影响因子：
7.2
通讯作者：
Sauer, N
中科院分区：
文献类型：
--
作者：
Gahrtz, M;Schmelzer, E;Sauer, N
车前草质膜蔗糖-Ht同向转运体PmSUC 1的cDNA克隆已在酿酒酵母中分离和表达。PmSUC 1蛋白的特点是在转基因酵母和蛋白脂质体与人工质子动力（pmf）发电机。PmSUC 1在pmf存在下催化蔗糖或麦芽糖的主动摄取，并且对解偶联剂敏感。与极端pH依赖性的PmSUC 2蔗糖-H+同向转运体不同，PmSUC 1对胞外pH的变化相对不敏感。在大叶黄杨的叶片和叶柄中，PmSUC 1 mRNA的表达仅限于维管系统。关于PmSUC 1的重要新特征是在大花牡丹花的非维管组织中发现最高的mRNA水平，其中该基因在种子发育的早期阶段瞬时表达。在现场杂交实验表明，PmSUC 1只在年轻的胚珠中表达; PmSUC 1的假定的生理作用进行了讨论。
A cDNA clone of the plasma membrane sucrose-Ht symporter PmSUC1 from Plantago major L. has been isolated and expressed in Saccharomyces cerevisiae. The PmSUC1 protein was characterized in transgenic yeast and in proteoliposomes with an artificial proton-motive-force (pmf) generator. PmSUC1 catalyzes the active uptake of sucrose or maltose in the presence of pmf and is sensitive to uncouplers. Unlike the extremely pH-dependent PmSUC2 sucrose-H+ symporter, PmSUC1 is relatively insensitive to changes of the extracellular pH. In leaves and petioles of P. major, expression of PmSUC1 mRNA is restricted to the vascular system. The important new feature about PmSUC1 is that the highest mRNA levels are found in non-vascular tissue of P. major flowers where the gene is transiently expressed during the early stages of seed development. In site hybridization experiments show that PmSUC1 is expressed only in young ovules; the putative physiological role of PmSUC1 is discussed.