Quinoline compounds decrease in vitro spontaneous proliferation of peripheral blood mononuclear cells (PBMC) from human T-cell lymphotropic virus (HTLV) type-1-infected patients
Quinoline compounds decrease in vitro spontaneous proliferation of peripheral blood mononuclear cells (PBMC) from human T-cell lymphotropic virus (HTLV) type-1-infected patients
复制标题
DOI：
10.1016/j.biopha.2007.05.003
复制
发表时间：
2008-09-01
影响因子：
7.5
通讯作者：
Foumet, Alain
中科院分区：
文献类型：
--
作者：
Grassi, Femanda;Correa, Ana Beatriz Guimaraes;Foumet, Alain
In vitro spontaneous proliferation is the immunological hallmark of peripheral blood mononuclear cells (PBMC) from HTLV-1-infected individuals. Quinoline compounds down regulate in vitro cell proliferation of HTLV-1 transformed cell lines. In the present study we assessed the capacity of quinolines to inhibit spontaneous cell proliferation of PBMC from HTLV-1-infected individuals. Twenty-two quinolines were evaluated. Toxicity was first assessed on PBMC from healthy donors by using both the Trypan blue technique and Tetrazolium Salt (XTT) method and then the antiproliferative effect was measured by a classic lymphoproliferative assay on PBMC from three HTLV-1-infected individuals, in the presence of decreasing concentrations of quinolines (from 100 mu M to 0.8 mu M), after 5 days of culture. We found that 14 out of 22 compounds were non-toxic to PBMC from uninfected individuals at 100, 50 and 10 mu M. Four compounds presented a capacity to inhibit more than 80% of the spontaneous proliferation: 7 at 25 mu M and 10, 20 and 23 at 100 mu M. Our results indicate that some quinolines block spontaneous proliferation of PBMC from HTLV-1-infected individuals. (C) 2007 Elsevier Masson SAS. All rights reserved.
