Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy
Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy
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DOI：
10.1016/j.bcmd.2009.01.018
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发表时间：
2009-05-01
影响因子：
2.3
通讯作者：
Marquet, Pierre
中科院分区：
文献类型：
--
作者：
Rappaz, Benjamin;Barbul, Alexander;Marquet, Pierre
红细胞（RBC）膜的波动提供了重要的见解细胞状态。我们提出了一个空间分析的红细胞膜波动，使用数字全息显微镜（DHM）。这种干涉和无染料的技术，具有纳米轴向和微秒的时间灵敏度，使测量细胞膜波动（CMF）的整个细胞表面。DHM采集与允许提取膜波动幅度的模型相结合，同时考虑细胞膜拓扑结构。观察到CMF振幅在RBC表面上的不均匀分布，在对应于RBC环面上的最高点的环中以及在RBC内部区域中的一些分散区域中显示最大值。正常和乙醇固定RBC的CMF振幅分别为35.9 +/- 8.9 nm和4.7 +/- 0.5 nm（细胞表面平均值）。(C)2009 Elsevier Inc. All rights reserved.
Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic Microscopy (DHM). This interferometric and dye-free technique, possessing nanometric axial and microsecond temporal sensitivities enables to measure cell membrane fluctuations (CMF) on the whole cell surface. DHM acquisition is combined with a model which allows extracting the membrane fluctuation amplitude, while taking into account cell membrane topology. Uneven distribution of CMF amplitudes over the RBC surface is observed, showing maximal values in a ring corresponding to the highest points on the RBC torus as well as in some scattered areas in the inner region of the RBC. CMF amplitudes of 35.9 +/- 8.9 nm and 4.7 +/- 0.5 nm (averaged over the cell surface) were determined for normal and ethanol-fixed RBCs, respectively. (C) 2009 Elsevier Inc. All rights reserved.