Immunomodulatory properties of captopril, an ACE inhibitor, on LPS-induced lung inflammation and fibrosis as well as oxidative stress
Immunomodulatory properties of captopril, an ACE inhibitor, on LPS-induced lung inflammation and fibrosis as well as oxidative stress
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DOI：
10.1007/s10787-018-0535-4
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发表时间：
2019-06-01
影响因子：
5.8
通讯作者：
Boskabady, Mohammad Hossein
中科院分区：
文献类型：
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作者：
Boskabadi, Javad;Askari, Vahid Reza;Boskabady, Mohammad Hossein
背景血管紧张素转换酶抑制剂在炎症过程中的作用已被证实。方法采用内毒素（LPS）致大鼠肺损伤模型，观察对照组、内毒素（LPS）组、内毒素致伤前给予12.5、25、50 mg/kg卡托普利组和内毒素致伤前给予50 mg/kg卡托普利组的肺损伤情况。结果LPS组MDA含量、LPS+ Cap 50组WBC总数、LPS+ Cap 25和LPS+ Cap 50组中性粒细胞百分率、LPS+ Cap 25和LPS+ Cap 50组IFN-、PGE 2、TGF-1和IL-4水平、SOD和CAT活性、MDA含量、总巯基含量、TNF-α、TGF-1和IL-4水平均明显高于对照组。LPS + Cap 50组TGF-1和LPS+ Cap 25、LPS+ Cap 50组IFN-1/IL-4比值均显著低于LPS组（P < 0. 05），而LPS+ Cap 25、LPS+ Cap 50组总巯基、SOD活性、淋巴细胞百分率、CAT活性和IL-4浓度均显著高于LPS组（P < 0. 05）。
BackgroundThe role of angiotensin converting enzyme inhibitors on the inflammation process has been demonstrated previously. In the present study, the effects of captopril on lung injury induced by lipopolysaccharide (LPS) were investigated.MethodsControl, LPS, 12.5, 25 and 50mg/kg captopril-treated before LPS administration and captopril 50mg/kg before saline administration groups of rats were studied. Total and percentage of differential WBC, the levels of MDA, total thiol groups, the activities of SOD and CAT, the levels of IFN-, PGE2, TGF-1 and IL-4 in the BALF were evaluated.ResultsMDA concentration in LPS groups treated with all captopril concentrations, total WBC in LPS+Cap50, percent of neutrophils in LPS+Cap25 and LPS+Cap50, levels of IFN-, PGE2, TGF-1 in LPS+Cap50 and IFN-/IL-4 ratio in LPS+Cap25 and LPS+Cap50 were significantly decreased but total thiol groups and activity of SOD in LPS+Cap25 and LPS+Cap50, percent of lymphocyte, CAT activity and concentration of IL-4 only in LPS+Cap50 group were increased in comparison to the LPS group (p