GRANULA MOTION AND MEMBRANE SPREADING DURING ACTIVATION OF HUMAN PLATELETS IMAGED BY ATOMIC-FORCE MICROSCOPY
GRANULA MOTION AND MEMBRANE SPREADING DURING ACTIVATION OF HUMAN PLATELETS IMAGED BY ATOMIC-FORCE MICROSCOPY
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DOI：
10.1016/s0006-3495(94)80963-4
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发表时间：
1994-05-01
影响因子：
3.4
通讯作者：
GAUB, HE
中科院分区：
文献类型：
--
作者：
FRITZ, M;RADMACHER, M;GAUB, HE
活化过程中血小板成分的重新分布对于维持止血的生理功能至关重要。我们在这里报告约真实的时间调查的激活天然人类血小板在生理条件下，从最初形成的丝状伪足的完全传播的形式，通过原子力显微镜。我们跟踪了颗粒的运输及其与单细胞内质膜的相互作用。我们的研究结果表明，某些颗粒向板状伪足的运动。对这种重排和随后的血小板表面扩大的分析揭示了膜扩展过程的细节。活细胞的图像显示的细胞骨架成分和膜结合丝的分布在一个分辨率优于50纳米。尖端和血小板之间的局部最小力估计小于60 pN。利用接枝聚合物的理论，开发了一个模型的弹性贡献的glycocalix的尖端/膜的相互作用。
The redistribution of platelet constituents during activation is essential for their physiological function of maintaining hemostasis. We report here about real time investigations of the activation of native human platelets under physiological conditions from the initial formation of filopodia to the fully spread form by atomic force microscopy. We followed the trafficking of granules and their interaction with the plasma membrane within single cells. Our results show movement of certain granula towards the lamellipodia. Analysis of this rearrangement and the subsequent enlargement of the platelet surface reveals details of the membrane spreading process. Images of living cells are presented that show the distribution of cytoskeletal components and membrane-bound filaments at a resolution of better than 50 nm. The local minimum forces between the tip and the platelets were estimated to be smaller than 60 pN. A model for the elastic contributions of the glycocalix to the tip/membrane interaction was developed using the theory of grafted polymers.