Mechanical quality of tissue engineered cartilage:: Results after 6 and 12 weeks in vivo
Mechanical quality of tissue engineered cartilage:: Results after 6 and 12 weeks in vivo
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DOI：
10.1002/1097-4636(2000)53:6
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发表时间：
2000-12-05
期刊：
影响因子：
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通讯作者：
Sittinger, M
中科院分区：
文献类型：
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作者：
Duda, GN;Haisch, A;Sittinger, M
创伤性事件是局部关节软骨病变的主要原因。如果及早治疗，可以恢复初始关节的几何形状和完整性。在大的缺陷中，没有足够的材料来桥接受影响的区域。由于感染和免疫反应的风险，异种移植不被广泛接受。另一种选择是软骨样结构，可以在体外培养并移植到缺损部位。这些新组织成功的关键在于它们的机械性能。本研究的目的是生成透明样软骨结构，评估其在体内的性能，并验证其细胞和材料性能符合天然软骨的性能。体外生成透明样软骨标本，植入裸鼠背部。标本在6周和12周后外植，用压痕试验进行机械测试和组织学检查。在力学测试中，刚度和破坏载荷在第6周和第12周之间显著增加。12周时，透明状软骨的力学性能与天然鼻中隔软骨相当。与天然关节软骨相比，工程组织在强度和机械刚度方面达到了30-50%。组织学检查，标本显示新软骨形成。力学测试程序被证明是足够敏感的，可以识别不同来源和不同愈合阶段的软骨标本之间的特性差异。作为组织学分析的辅助手段，力学测试可能是在广泛临床应用之前判断工程软骨效用的有价值的工具。(C) 2000 John Wiley & Sons, Inc。
Traumatic events are a primary cause for local lesions of articular cartilage. If treated early, restoration of the initial joint geometry and integrity may be achieved. In large defects, sufficient material is not available to bridge the affected area. Heterologeous transplantation is not well accepted due to the risk of infection and immune response. Alternatives are cartilage-like structures, which may be cultured kt vitro and transplanted into the defect site. Critical to the success of these new tissues are their mechanical properties. Goals of this study were to generate a hyaline-like cartilage structure, to evaluate its performance in vivo and to verify that its cellular and material properties meet those of native cartilage. Hyaline-like cartilage specimens were generated in vitro and implanted in the backs of nude mice. Specimens were explanted after 6 and 12 weeks, mechanically tested using an indentation test and histologically examined. In mechanical testing, stiffness and failure load significantly increased between weeks 6 and 12. At 12 weeks, mechanical properties of the hyaline-like cartilage were comparable to those of native nasal septal cartilage. Compared to native articular cartilage, the engineered tissue achieved up to 30-50% in strength and mechanical stiffness. In histological examination, specimens showed neocartilage formation. The mechanical testing procedure proved to be sufficiently sensitive to identify differences in properties between cartilage specimens of different origin and at different stages of healing. As an adjunct to histological analysis, mechanical testing may be a valuable tool for judging the utility of engineered cartilage prior to a broad clinical usage. (C) 2000 John Wiley & Sons, Inc.