Kinetic Studies of Paclitaxel Production by Taxus canadensis Cultures in Batch and Semicontinuous with Total Cell Recycle
Kinetic Studies of Paclitaxel Production by Taxus canadensis Cultures in Batch and Semicontinuous with Total Cell Recycle
复制标题
加拿大红豆杉培养物分批和半连续全细胞回收紫杉醇生产的动力学研究
DOI：
--
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
J. Linden
中科院分区：
文献类型：
--
作者：
M. Phisalaphong;J. Linden
在规定的顶空乙烯浓度下，用茉莉酸甲酯(MJ)诱导加拿大红豆杉悬浮培养。在分批培养和全细胞循环的半连续培养条件下，对紫杉醇的生长、养分消耗、pH变化和紫杉醇积累进行了动力学研究。用10 0μM MJ诱导后，紫杉醇含量显著增加，但细胞生长受阻。添加乙酰辅酶A和MJ是提高紫杉醇产量的另一种途径。采用全细胞循环的半连续培养，紫杉醇的积累在35d内比分批培养提高了4.0倍。
Suspension cultures of Taxus canadensis were elicited with methyl jasmonate (MJ) under defined headspace ethylene concentrations. Kinetic studies of growth, nutrient consumption, pH variation, and paclitaxel accumulation were conducted in batch cultures and semicontinuous culture with total cell recycle. A dramatic increase of paclitaxel was obtained when the cultures were elicited with 100 μM MJ, but cell growth was thereby arrested. Supplementation of acetyl‐CoA and MJ to the culture proved to be another way to improve paclitaxel yields. Using semicontinuous culture with total cell recycle, paclitaxel accumulation was increased by a factor of 4.0 relative to that in the batch culture during 35 days of cultivation.
大规模昆虫和植物细胞培养。
影响因子：
7.7
作者：
Taticek,RA;Lee,CW;Shuler,ML
通讯作者：
Shuler,ML