DNA-POLYMERASE-I - ESSENTIAL REPLICATION ENZYME
DNA-POLYMERASE-I - ESSENTIAL REPLICATION ENZYME
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DOI：
10.1126/science.781842
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发表时间：
1976-01-01
期刊：
影响因子：
56.9
通讯作者：
UYEMURA, DG
中科院分区：
文献类型：
--
作者：
LEHMAN, IR;UYEMURA, DG
综述了DNA聚合酶I在大肠杆菌染色体复制中的作用。编码DNA聚合酶I的基因(Po1A)突变在体内的后果与该突变对突变改变的酶的物理和催化特性的影响是相关的。讨论了po1A基因两个非致命性突变po1A1和po1A12产生的酶缺陷的性质，以及条件致死性突变po1Aex1造成的缺陷。涵盖的主题包括聚合酶作用的机制、DNA聚合酶I在解释DNA复制方面的不足、发现凯恩斯突变和DNA聚合酶I的抑制作用、非致命性DNA聚合酶I突变、po1A12突变和条件致死DNA聚合酶I突变。
The role of DNA polymerase I in the replication of the Escherichia coli chromosome was summarized. The consequences in vivo of a mutation in the gene (po1A) that codes for DNA polymerase I with the effect of that mutation on the physical and catalytic properties of the mutationally altered enzyme are correlated. The nature of the enzymatic defect produced by 2 nonlethal mutations in the po1A gene, po1A1 and po1A12; and the defect resulting from the conditionally lethal mutation, po1Aexl, are discussed. Topics covered include the mechanism of polymerase action, the inadequacy of DNA polymerase I in accounting for DNA replication, the discovery of the Cairns mutant and repressed role of DNA polymerase I, nonlethal DNA polymerase I mutants, the po1A12 mutant and conditionally lethal DNA polymerase I mutants.