Human memory T cell responses to SARS-CoV E protein
Human memory T cell responses to SARS-CoV E protein
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DOI：
10.1016/j.micinf.2006.05.008
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发表时间：
2006-08-01
影响因子：
5.8
通讯作者：
Wu, Chang-you
中科院分区：
文献类型：
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作者：
Peng, Hui;Yang, Li-tao;Wu, Chang-you
E蛋白是严重急性呼吸综合征冠状病毒（SARS-CoV）的膜组分。E蛋白的破坏可能会降低病毒的传染性。因此，SARS-CoV E蛋白被认为是开发抗病毒药物的潜在靶标。然而，人体对E蛋白的细胞免疫反应尚不清楚。在这项研究中，我们发现来自完全康复的SARS个体的外周血单核细胞（PBMCs）在用重叠整个E蛋白序列的9个肽池刺激后迅速产生ifn - γ和IL-2。流式细胞术分析免疫应答结果显示，CD4（+）和CD8(+)T细胞均参与了SARS-CoV E肽刺激后的SARS-CoV E特异性免疫应答。此外，大多数ifn - γ (+)CD4(+)T细胞是表达CD45RO(+)CCR7(+)CD62L（-）的中枢记忆细胞；而ifn - γ (+)CD8（+）记忆T细胞主要是表达CD45RO(-)CCR7(-)CD62L（-）的效应记忆细胞。T细胞对9个肽段的应答结果表明，E蛋白含有至少两个主要的T细胞表位（E2氨基酸[aa] 9-26和E5-6: aa 33-57），它们在引发人对SARS-CoV E蛋白的细胞免疫应答中起重要作用。(c) 2006 Elsevier SAS。版权所有。
E protein is a membrane component of severe acute respiratory syndrome coronavirus (SARS-CoV). Disruption of E protein may reduce viral infectivity. Thus, the SARS-CoV E protein is considered a potential target for the development of antiviral drugs. However, the cellular immune responses to E protein remain unclear in humans. In this study, we found that peripheral blood mononuclear cells (PBMCs) from fully recovered SARS individuals rapidly produced IFN-gamma and IL-2 following stimulation with a pool of 9 peptides overlapping the entire E protein sequence. Analysis of the immune responses by flow cytometry showed that both CD4(+) and CD8(+)T cells were involved in the SARS-CoV E-specific immune responses after stimulation with SARS-CoV E peptides. Moreover, the majority of IFN-gamma(+)CD4(+)T cells were central memory cells expressing CD45RO(+)CCR7(+)CD62L(-); whereas IFN-gamma(+)CD8(+) memory T cells were mostly effector memory cells expressing CD45RO(-)CCR7(-)CD62L(-). The results of T-cell responses to 9 individual peptides indicated that the E protein contained at least two major T cell epitopes (E2 amino acid [aa] 9-26 and E5-6: aa 33-57) which were important in eliciting cellular immune response to SARS-CoV E protein in humans. (c) 2006 Elsevier SAS. All rights reserved.