Drosophila follicle cell amplicons as models for metazoan DNA replication: a cyclinE mutant exhibits increased replication fork elongation.
Drosophila follicle cell amplicons as models for metazoan DNA replication: a cyclinE mutant exhibits increased replication fork elongation.
复制标题
果蝇滤泡细胞扩增子作为后生动物 DNA 复制的模型：cyclinE 突变体表现出复制叉伸长增加。
DOI：
10.1073/pnas.0707804104
复制
发表时间：
2007
影响因子：
11.1
通讯作者：
Orr-Weaver,TerryL
中科院分区：
文献类型：
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作者：
Park,EugeniaA;Macalpine,DavidM;Orr-Weaver,TerryL
Gene clusters amplified in the ovarian follicle cells ofDrosophilaserve as powerful models for metazoan DNA replication. In response to developmental signals, specific genomic regions undergo amplification by repeated firing of replication origins and bidirectional movement of replication forks for ≈50 kb in each direction. Previous work focused on initiation of amplification, defining replication origins, establishing the role of the prereplication complex and origin recognition complex (ORC), and uncovering regulatory functions for the Myb, E2F1, and Rb transcription factors. Here, we exploit follicle cell amplification to investigate the control of DNA replication fork progression and termination, poorly understood processes in metazoans. We identified a mutant in which, during gene amplification, the replication forks move twice as far from the origin compared with wild type. This phenotype is the result of an amino acid substitution mutation in thecyclinEgene,cyclinE1f36. The rate of oogenesis is normal incyclinE1f36/cyclinEPz8mutant ovaries, indicating that increased replication fork progression is due to increased replication fork speed, possibly from increased processivity. The increased amplification domains observed in the mutant imply that there are not replication fork barriers preventing replication forks from progressing beyond the normal 100-kb amplified region. These results reveal a previously unrecognized role for CyclinE in controlling replication fork movement.
