Selection of microbial mutants tolerant to extreme environmental stress using continuous culture-control design
Selection of microbial mutants tolerant to extreme environmental stress using continuous culture-control design
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DOI：
10.1021/bp990084j
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发表时间：
1999-11-01
影响因子：
2.9
通讯作者：
Butler, PR
中科院分区：
文献类型：
--
作者：
Lane, PG;Hutter, A;Butler, PR
考虑了连续选择技术的控制器设计(BOICS；Brown and Oliver，1982)。这项技术被用来获得耐受极端环境胁迫的微生物突变体。由于控制器设计问题，BOICS的应用一直受到阻碍。在本文中，我们考虑了一种改进的BOICS实现，它具有许多实用的优点。采用了基于模型的控制器设计方法。考虑了压力是由于生长环境中的抑制物质造成的情况。该分析适用于有机体和抑制剂的任何合理组合。考虑了常规的线性控制器和时不变控制器。提出了控制器参数取值的指导原则。这些准则的应用要求确定某些工艺参数的值。提出了识别这些参数值的方法。仿真结果表明，所设计的控制器性能令人满意。模型选择实验的实验数据证实了这一点。控制器设计的配方是BOICS协议的必要部分。希望本文提供的控制器设计问题的解决方案将鼓励该技术的进一步应用。
The design of controllers for a continuous selection technique (BOICS; Brown and Oliver, 1982) is considered. This technique is used to obtain microbial mutants that are tolerant to extreme environmental stress. Applications of BOICS have been hampered by the problem of controller design. In this paper, a modified implementation of BOICS is considered which has a number of practical advantages. A model-based approach to controller design is taken. The case in which the stress is due to an inhibitory substance in the growth environment is considered. The analysis is intended to be applicable to any reasonable combination of organism and inhibitor. Conventional linear and time-invariant controllers are considered. Guidelines for the selection of controller parameters' values are suggested. The application of these guidelines requires that certain process parameters' values be identified. Methods by which these parameters' values can be identified are suggested. Simulation results indicate that the resulting controllers perform satisfactorily. This is confirmed by experimental data from a model selection experiment. A recipe for the design of controllers is a necessary part of a protocol for BOICS. It is hoped that the solution to the controller design problem that is offered in this paper will encourage further applications for the technique.