Improved chemical stability and cellular antioxidant activity of resveratrol in zein nanoparticle with bovine serum albumin-caffeic acid conjugate
Improved chemical stability and cellular antioxidant activity of resveratrol in zein nanoparticle with bovine serum albumin-caffeic acid conjugate
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牛血清白蛋白-咖啡酸缀合物改善了玉米醇溶蛋白纳米颗粒中白藜芦醇的化学稳定性和细胞抗氧化活性
DOI：
10.1016/j.foodchem.2018.04.055
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发表时间：
2018-09-30
期刊：
影响因子：
8.8
通讯作者：
Yi, Jiang
中科院分区：
文献类型：
--
作者：
Fan, Yuting;Liu, Yuexiang;Yi, Jiang
采用自由基接枝法制备了牛血清白蛋白（BSA）-咖啡酸（CA）偶联物。缀合物的CA与BSA的比率为115.7mg/g。体外抗氧化活性实验表明，BSA-CA偶联物具有比BSA更强的抗氧化活性。采用抗溶剂法制备了白藜芦醇玉米醇溶蛋白-BSA和BSA-CA核壳复合纳米粒。BSA和BSA-CA的粒径分别为206.3 nm和217.2 nm。玉米醇溶蛋白-BSA和玉米醇溶蛋白-BSA-CA纳米颗粒的包封率（EE）分别为85.3%和86.5%。SEM结果表明，两种纳米粒子均为球形，平均粒径约为200 nm，表面光滑。纳米胶囊化后白藜芦醇的热稳定性和紫外光稳定性均得到显著提高。BSA-CA偶联物对白藜芦醇降解的保护作用明显强于BSA。细胞抗氧化活性（CAA）研究证实，玉米醇溶蛋白-BSA和玉米醇溶蛋白-BSA-CA纳米粒中的白藜芦醇比单独的白藜芦醇具有显着更高的抗氧化活性。
In this study, bovine serum albumin (BSA)-caffeic acid (CA) conjugate was prepared with free radical-induced grafting method. The CA to BSA ratio of the conjugate was 115.7 mg/g. In vitro antioxidant activity assays suggested that BSA-CA conjugates had stronger antioxidant activity than BSA. Resveratrol-loaded zein encapsulated with BSA and BSA-CA conjugate core-shell nanoparticles were prepared with antisolvent method. Particle sizes were 206.3 nm, and 217.2 nm for BSA and BSA-CA, respectively. The encapsulation efficiencies (EEs) were 85.3% and 86.5% for zein-BSA and zein-BSA-CA nanoparticles, respectively. SEM results indicated that both nanoparticles were spherical with mean diameter approximately 200 nm and smooth surfaces. Both thermal and UV light stability of resveratrol was significantly improved after nanoencapsulation. BSA-CA conjugate showed remarkably greater protection than BSA against resveratrol degradation. Cellular antioxidant activity (CAA) study confirmed that resveratrol in both zein-BSA and zein-BSA-CA nanoparticles had significant higher antioxidant activities than resveratrol alone.