Microwave fixation: Its potential for routine techniques, histochemistry, immunocytochemistry and electron microscopy
Microwave fixation: Its potential for routine techniques, histochemistry, immunocytochemistry and electron microscopy
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微波固定：其在常规技术、组织化学、免疫细胞化学和电子显微镜方面的潜力
DOI：
10.1007/bf01003442
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
M. Kerr
中科院分区：
文献类型：
--
作者：
D. Hopwood;G. Coghill;J. Ramsay;G. Milne;M. Kerr
人体组织（活组织检查和死后组织）以及啮齿动物组织在不同温度下通过微波固定，并与甲醛固定材料进行比较。传统染色剂，包括三色染色剂，效果很好。红细胞被裂解，但白细胞被固定，从而可以诊断各种炎症状态。两种方法对恶性细胞的保存同样完好。粘液物质、脂质和各种水解酶的组织化学研究显示两种技术之间没有显着差异。然而，一些神经染色在微波处理后效果不佳。与甲醛固定的组织相比，微波固定后 IgA、IgM 和 IgG 的免疫细胞化学定位没有显着差异。微波固定不会导致比甲醛固定更大的组织收缩。两者均显着低于单独使用磷酸盐缓冲盐水处理后的结果。电子显微镜给出的结果是可以解释的，但损害类似于早期死后的变化。微波固定大约在 1-2 分钟内完成。固定机制似乎是由于与二硫键形成相关的变性和蛋白质溶解度的降低。
Human tissues, both biopsy and postmortem, and tissues from rodents were fixed by microwaves at various temperatures and compared against formaldehyde-fixed material. Conventional stains, including trichromes, worked well. Red cell were lysed, but white cells were fixed, thus permitting diagnoses of various inflammatory states. Malignant cells were equally well-preserved by the two methods. Histochemical investigations of mucosubstances, lipids and various hydrolases showed no significant difference between the two techniques. Some neurological stains, however, were not as good following microwave treatment. Immunocytochemical localization of IgA, IgM and IgG showed no significant difference after microwave fixation compared to that in tissues fixed with formaldehyde. Microwave fixation did not lead to a greater tissue shrinkage than that obtained with formaldehyde fixation. Both were significantly less than that following treatment with phosphate-buffered saline alone. Electron microscopy gave results which were interpretable, but with damage resembling early postmortem change. Microwave fixation is complete in approximately 1–2 min.The mechanism of fixation appears to be due to denaturation associated with disulphide bond formation and a decrease in solubility of proteins.