Acinetobactin-Mediated Inhibition of Commensal Bacteria by Acinetobacter baumannii.
Acinetobactin-Mediated Inhibition of Commensal Bacteria by Acinetobacter baumannii.
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不动杆菌素介导的鲍曼不动杆菌对共生菌的抑制作用
DOI：
10.1128/msphere.00016-22
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发表时间：
2022-02-23
期刊：
影响因子：
4.8
通讯作者：
Davies BW
中科院分区：
文献类型：
--
作者：
Knauf GA;Powers MJ;Herrera CM;Trent MS;Davies BW
鲍曼不动杆菌是一种重要的医院相关病原体，可引起抗生素耐药感染和反复发生的医院暴发。鲍曼不动杆菌无症状定植患者的能力是感染的危险因素，并加剧了其传播。然而，很少有信息描述它用来殖民病人的机制。鲍曼不动杆菌常寄生在上呼吸道和皮肤上。抗生素的使用是定植和感染的危险因素，这表明鲍曼不动杆菌可能与共生细菌竞争以建立一个生态位。为了开始研究这种可能性，我们将鲍曼不动杆菌与上呼吸道和皮肤的共生细菌共培养。在模拟宿主铁饥饿的条件下，我们观察到鲍曼不动杆菌抑制表皮葡萄球菌、人型葡萄球菌、溶血葡萄球菌和纹状棒状杆菌。然后利用有序转座子文库筛选鲍曼不动蛋白，确定鲍曼不动蛋白是抑制表型的致病因子。通过质谱分析，我们发现在我们的共培养条件下，鲍曼不动杆菌蛋白可以从鲍曼不动杆菌中释放出来，纯化后的不动杆菌蛋白可以抑制纹状梭菌和人杆菌。综上所述，我们的数据表明，不动杆菌蛋白可能为鲍曼不动杆菌提供了一种竞争优势，而不是一些呼吸道和皮肤共生细菌，并可能支持其定植患者的能力。鲍曼不动杆菌无症状定植患者的能力是感染的危险因素，并加剧了其临床传播。然而，很少有信息描述鲍曼不动杆菌如何无症状地定殖患者。本研究提供证据表明鲍曼不动杆菌可以通过铁竞争抑制多种皮肤和上呼吸道共生细菌的生长，并确定不动杆菌蛋白是支持其营养优势的分子。通过铁竞争战胜内源性共生体可能支持鲍曼不动杆菌在患者中定植和传播的能力。
Acinetobacter baumannii is an important hospital-associated pathogen that causes antibiotic resistant infections and reoccurring hospital outbreaks. A. baumannii’s ability to asymptomatically colonize patients is a risk factor for infection and exacerbates its spread. However, there is little information describing the mechanisms it employs to colonize patients. A. baumannii often colonizes the upper respiratory tract and skin. Antibiotic use is a risk factor for colonization and infection suggesting that A. baumannii likely competes with commensal bacteria to establish a niche. To begin to investigate this possibility, we cocultured A. baumannii and commensal bacteria of the upper respiratory tract and skin. In conditions that mimic iron starvation experienced in the host, we observed that A. baumannii inhibits Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus haemolyticus and Corynebacterium striatum. Then using an ordered transposon library screen we identified the A. baumannii siderophore acinetobactin as the causative agent of the inhibition phenotype. Using mass spectrometry, we show that acinetobactin is released from A. baumannii under our coculture conditions and that purified acinetobactin can inhibit C. striatum and S. hominis. Together our data suggest that acinetobactin may provide a competitive advantage for A. baumannii over some respiratory track and skin commensal bacteria and possibly support its ability to colonize patients. IMPORTANCE The ability of Acinetobacter baumannii to asymptomatically colonize patients is a risk factor for infection and exacerbates its clinical spread. However, there is minimal information describing how A. baumannii asymptomatically colonizes patients. Here we provide evidence that A. baumannii can inhibit the growth of many skin and upper respiratory commensal bacteria through iron competition and identify acinetobactin as the molecule supporting its nutritional advantage. Outcompeting endogenous commensals through iron competition may support the ability of A. baumannii to colonize and spread among patients.
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DOI：
10.1186/cc11383
发表时间：
2012-06-13
期刊：
Critical care (London, England)
影响因子：
--
作者：
Arvaniti K;Lathyris D;Ruimy R;Haidich AB;Koulourida V;Nikolaidis P;Matamis D;Miyakis S
通讯作者：
Miyakis S
影响因子：
4.4
作者：
Eijkelkamp BA;Hassan KA;Paulsen IT;Brown MH
通讯作者：
Brown MH
DOI：
10.1038/nrmicro2259
发表时间：
2010-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
9.4
作者：
Marchaim, Dror;Navon-Venezia, Shiri;Carmeli, Yehuda
通讯作者：
Carmeli, Yehuda
影响因子：
30.3
作者：
Deriu E;Liu JZ;Pezeshki M;Edwards RA;Ochoa RJ;Contreras H;Libby SJ;Fang FC;Raffatellu M
通讯作者：
Raffatellu M