Establishment of a human endometrial cell culture system and characterization of its polarized hormone responsive epithelial cells
Establishment of a human endometrial cell culture system and characterization of its polarized hormone responsive epithelial cells
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DOI：
10.1007/s004410050743
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发表时间：
1996-12
影响因子：
3.6
通讯作者：
I. Classen-Linke;M. Kusche;R. Knauthe;H. M. Beier
中科院分区：
文献类型：
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作者：
I. Classen-Linke;M. Kusche;R. Knauthe;H. M. Beier
在双室系统中培养来自正常人子宫内膜的子宫上皮细胞作为原代细胞培养物。上皮细胞分离自子宫切除术获得的增殖期子宫内膜组织。将上皮细胞接种在涂覆有细胞外基质Matrigel的Millicell CM滤器上。取决于培养条件，上皮细胞在Matrigel上形成极化细胞单层或在Matrigel中形成腺样结构。电镜观察显示，培养过程中上皮细胞极性保持不变。孕激素和雌激素受体作为生理完整的子宫内膜上皮细胞的典型标志分子，可在体外用免疫组化和RT-PCR方法检测到，并被作为孕酮类似物的醋酸甲羟孕酮（MPA）下调。由于该细胞培养系统具有典型的体内情况的形态学和免疫组织化学特征，并且由于它可以在所描述的体外条件下通过激素处理来调节，因此它代表了用于研究子宫内膜分化和生殖功能所必需的过程的有价值的工具。
Uterine epithelial cells from normal human endometrium were cultured as a primary cell culture in a dual-chambered system. The epithelial cells were isolated from endometrial tissue of the proliferative phase obtained by hysterectomy. The epithelial cells were seeded on Millicell CM filters coated with the extracellular matrix Matrigel. Depending on the culture conditions, the epithelial cells formed a polarized cell monolayer on Matrigel or gland-like structures in Matrigel. The epithelial cell polarity was maintained during culture, which could be proved by electron microscopy. The progesterone and estrogen receptors as typical marker molecules for physiologically intact endometrial epithelial cells could be detected immunohistochemically as well as by RT-PCR in vitro and were down-regulated by medroxyprogesterone acetate (MPA) used as progesterone analogue. As this cell culture system exhibits morphological and immunohistochemical characteristics, typical for the in vivo situation, and since it can be modulated by hormone treatment under the in vitro conditions described, it represents a valuable tool for investigating processes that are essential for endometrial differentiation and reproductive functions.