A primer on single-cell genomics in myeloid biology.
A primer on single-cell genomics in myeloid biology.
复制标题
DOI：
10.1097/moh.0000000000000623
复制
发表时间：
2021-01
影响因子：
3.2
通讯作者：
Leighton Grimes, H.
中科院分区：
文献类型：
--
作者：
Ferchen, Kyle;Song, Baobao;Leighton Grimes, H.
理解快速发展的单细胞技术领域，如应用于骨髓生物学，需要基本的分子，信息学和生物学概念的升值。在这里，我们强调了关键和最近的手稿，以说明那些新的骨髓血液学分子单细胞分析的基本概念。最近的研究应用单细胞组学发现新的细胞群，构建细胞群之间的关系，重新配置造血组织，研究造血谱系树和命运选择。随着技术的发展，新的信息工具已经出现，提供了令人兴奋的新见解。造血干细胞和祖细胞（HSPCs）受复杂的内在和外在因素的调节，以产生血细胞类型。在这篇综述中，我们讨论了单细胞组学的最新进展，这些细胞，方法来推断细胞类型识别和轨迹从分子组学数据，最终得出新的见解HSPC生物学。我们进一步讨论了这些技术的未来应用，以了解造血细胞的相互作用，功能和发展。我们的目标是提供一个全面的概述，目前的单细胞技术和他们的影响，我们的理解骨髓细胞的发展，为那些新的单细胞分析。
Understanding the fast-moving field of single-cell technologies, as applied to myeloid biology, requires an appreciation of basic molecular, informatics and biological concepts. Here, we highlight both key and recent manuscripts to illustrate basic concepts for those new to molecular single-cell analyses in myeloid hematology. Recent studies apply single-cell omics to discover novel cell populations, construct relationships between cell populations, re-configure the organization of hematopoiesis, and study hematopoietic lineage tree and fate choices. Accompanying development of technologies, new informatic tools have emerged, providing exciting new insights. Hematopoietic stem and progenitor cells (HSPCs) are regulated by complex intrinsic and extrinsic factors to produce blood cell types. In this review we discuss recent advances in single cell omics to profile these cells, methods to infer cell type identify and trajectories from molecular omics data to ultimately derive new insights into HSPC biology. We further discuss future applications of these technologies to understand hematopoietic cell interactions, function and development. The goal is to offer a comprehensive overview of current single cell technologies and their impact on our understanding of myeloid cell development for those new to single cell analyses.