Adhesion and Proliferation of Human Adipose-Derived Stem Cells on Titania Nanotube Surfaces
Adhesion and Proliferation of Human Adipose-Derived Stem Cells on Titania Nanotube Surfaces
复制标题
DOI：
10.1007/s40883-019-00091-9
复制
发表时间：
2019-12-01
影响因子：
2.6
通讯作者：
Popat, Ketul C.
中科院分区：
文献类型：
--
作者：
Cowden, Kari;Dias-Netipanyj, Marcela Ferreira;Popat, Ketul C.
当植入生物材料时，身体的反应类似于损伤，干细胞被招募到植入部位。由于干细胞在体内组织修复中发挥着重要作用，因此它们与生物材料的相互作用对于医疗器械的长期成功至关重要。具有纳米结构特征的表面已被证明在体外改变细胞功能，甚至在体内改善植入物与周围骨组织的固定。在这项研究中，二氧化钛纳米管（NT）的大小和细胞密度对人脂肪干细胞（ASCs）的粘附和增殖的影响进行了评估。虽然许多研究已经评估了间充质干细胞在纳米结构表面上的反应，但很少有研究探索了脂肪来源的干细胞在钛纳米管表面上的反应，并且没有研究探索了细胞密度与纳米管尺寸对ASC增殖的影响。使用三种不同的细胞密度研究了NT表面和钛对照上ASC的增殖行为一周。最佳细胞密度为2500个细胞/孔，直径较小的NT显示出较高的ASC增殖。这项研究证实，NT表面促进ASC粘附和增殖。通过更全面地了解纳米结构尺寸对干细胞粘附和增殖的影响，可以专门设计植入物，以实现植入组织的最佳干细胞反应。此外，ASC对这些NT表面的有利反应和最佳细胞密度的确定表明在骨科组织再生中的潜在用途。当生物材料被植入时，身体的反应类似于损伤，干细胞被募集到植入部位。由于干细胞在体内组织修复中发挥着重要作用，因此它们与生物材料的相互作用对于医疗器械的长期成功至关重要。这项研究证实，纳米管表面促进干细胞粘附和增殖。
When a biomaterial is implanted, the body reacts similar to an injury and stem cells are recruited to the implant site. Since, stem cells play an important role in tissue repair in the body, their interaction with biomaterials is critical for long-term success of medical devices. Surfaces with nanostructured features have been shown to alter cellular functionality in vitro and even improve fixation of the implant to the surrounding bone tissues in vivo. In this study, the effect of titania nanotube (NT) size and cell density on the adhesion and proliferation of human adipose-derived stem cells (ASCs) was evaluated. Although many studies have evaluated mesenchymal stem cells response on nanostructured surfaces, very few studies have explored the response of adipose-derived stem cells on titanium nanotube surfaces and none have explored the effect of cell density concurrent with nanotube size on ASC proliferation. Proliferation behavior of ASCs on NT surfaces and titanium control were investigated for a week using three different cell densities. The optimal cell density was 2500 cells/well and the smaller diameter NT exhibited higher ASC proliferation. This study confirms that NT surfaces promote ASC adhesion and proliferation. By more fully understanding the effect of nanostructure size on adhesion and proliferation of stem cells, implants could be specifically designed to achieve the optimal stem cell response from the tissue in which they are implanted. Additionally, the favorable response of ASC to these NT surfaces and determination of optimal cell density suggests a potential use in orthopedic tissue regeneration. Lay Summary When a biomaterial is implanted, the body reacts similar to an injury and stem cells are recruited to the implant site. Since, stem cells play an important role in tissue repair in the body, their interaction with biomaterials is critical for long-term success of medical devices. This study confirms that nanotube surfaces promote stem cell adhesion and proliferation.