Facilitating the pickup of individual DNA molecules by AFM nanomanipulation with tips mechanically worn on bare mica
Facilitating the pickup of individual DNA molecules by AFM nanomanipulation with tips mechanically worn on bare mica
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DOI：
10.1002/jemt.21104
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发表时间：
2012-05
影响因子：
2.5
通讯作者：
Na Duan;F. Long;Xinyan Wang;Bin Li;Jun Hu;Yi Zhang
中科院分区：
文献类型：
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作者：
Na Duan;F. Long;Xinyan Wang;Bin Li;Jun Hu;Yi Zhang
针尖是提高原子力显微镜(AFM)纳米操纵从固体基质中提取单个DNA分子效率的关键因素之一。我们发现，在纳米操纵操作之前，在某些固体衬底上佩戴AFM针尖将大大提高拾取效率，这归因于穿戴后针尖与DNA分子的亲和力随着针尖半径的增加而增加。结果表明，裸云母比APTES改性云母在穿戴过程中更能保持针尖的清洁，这是AFM纳米操纵过程中DNA拾取的关键。Microsc.技术资源，2012年。©2011 Wiley期刊，Inc.
The tip is one of the critical factors to improve the efficiency in picking up individual DNA molecules from solid substrates based on atomic force microscope (AFM) nanomanipulation. We found that wearing AFM tips on certain solid substrates in advance to nanomanipulation operation would largely improve the pickup efficiency, which was ascribed to the increasing affinity of the tip to the DNA molecules along with the increase of the tip radius after wearing. It was demonstrated that bare mica was superior to APTES‐modified mica to keep the tip clean while wearing, which was crucial for DNA pickup during AFM nanomanipulation. Microsc. Res. Tech., 2012. © 2011 Wiley Periodicals, Inc.