Imaging the molecular signatures of apoptosis and injury with radiolabeled annexin V.
Imaging the molecular signatures of apoptosis and injury with radiolabeled annexin V.
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DOI：
10.1513/pats.200901-001aw
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发表时间：
2009-08-15
期刊：
影响因子：
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通讯作者：
Blankenberg, Francis G
中科院分区：
文献类型：
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作者：
Blankenberg, Francis G
膜联蛋白V是一种在人体中普遍存在的细胞内蛋白，具有多种有趣的特性，包括对被称为磷脂酰丝氨酸（PS）的膜结合组成阴离子磷脂的纳米摩尔亲和力。PS在凋亡细胞或生理应激细胞表面选择性表达。因此，放射标记形式的膜联蛋白V已在动物模型和人类I期和II期试验中使用，以确定该示踪剂是否可以用作非小细胞肺癌和非霍奇金淋巴瘤治疗效果的早期替代标记物。放射标记膜联蛋白V的许多其他肺部成像应用也是可能的，包括检测和监测各种疾病中的活动性肺部炎症和其他病理生理应激源。在这篇文章中，凋亡的显著分子特征（和其他形式的细胞死亡），允许成像放射标记膜联蛋白V将被讨论。NSCLC和非霍奇金淋巴瘤II期影像学试验的最新结果也将详细介绍。最后，将概述该示踪剂在其他肺部病变成像中的潜在未来应用。
Annexin V is a ubiquitous intracellular protein in humans that has a variety of intriguing characteristics, including a nanomolar affinity for the membrane-bound constitutive anionic phospholipid known as phosphatidylserine (PS). PS is selectively expressed on the surface of apoptotic or physiologically stressed cells. As such, radiolabeled forms of annexin V have been used in both animal models and human Phase I and Phase II trials to determine if this tracer can be employed as an early surrogate marker of therapeutic efficacy in NSCLC and non-Hodgkin's lymphoma. Many other pulmonary imaging applications of radiolabeled annexin V are also possible, including the detection and monitoring of active pulmonary inflammation and other pathophysiologic stressors in a variety of diseases. In this article, the salient molecular features of apoptosis (and other forms of cell death) that permits imaging with radiolabeled annexin V will be discussed. The latest results from Phase II imaging trials with NSCLC and non-Hodgkin's lymphoma will be also be detailed. Finally, the potential future application of this tracer for the imaging of other pulmonary pathologies will be outlined.