UNIFLAGELLAR MUTANTS OF CHLAMYDOMONAS - EVIDENCE FOR THE ROLE OF BASAL BODIES IN TRANSMISSION OF POSITIONAL INFORMATION
UNIFLAGELLAR MUTANTS OF CHLAMYDOMONAS - EVIDENCE FOR THE ROLE OF BASAL BODIES IN TRANSMISSION OF POSITIONAL INFORMATION
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DOI：
10.1016/0092-8674(82)90436-6
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发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
LUCK, DJL
中科院分区：
文献类型：
--
作者：
HUANG, B;RAMANIS, Z;LUCK, DJL
用ICR-191对莱茵衣藻137~菌株进行诱变后，分离到一系列单鞭毛突变体，表现出明显的位置表型。在被检查的细胞中，超过95%的细胞中没有发育的鞭毛与眼斑相顺。位置表型和单鞭毛表型都通过有丝分裂和减数分裂稳定地传递，在回交中减数分裂分离是两个突变体对两个野生型后代。其中四个突变体，onil，uni2，uni3和uni4，已经被广泛研究。它们似乎是单个基因座的等位基因或紧密连锁(50.06个图谱单位)。单鞭毛缺陷在不同生长条件下或在稳定的二倍体细胞中的特征表达表明，突变改变了顺眼点鞭毛中鞭毛的发育速度。电子显微镜研究表明，发育缺陷存在于基底。对代表16个连锁群的33个核标记的广泛重组分析未能建立连锁。然而，UNI突变体显示出与四个未定位突变基因座的连锁。每个基因座的突变也会影响鞭毛的组装。
A series of uniflagellar mutants isolated following mutagenesis of Chlamydomonas reinhardtii (strain 137~) with ICR-191 show a remarkable positional phenotype. The flagellum that fails to develop is cis to the eyespot in more than 95% of the cells examined. Both the positional and the uniflagellar phenotypes are transmitted stably through mitotic and meiotic divisions, and in backcrosses the meiotic segregation is two mutant to two wild-type progeny. Four of the mutants, onil, uni2, uni3 and uni4, have been studied extensively. They appear to be alleles of a single gene locus or to be closely linked (50.06 map units). The characteristic expression of the uniflagellar defect in cells under different growth conditions or in stable diploids indicates that the mutations alter the rate of development of the flagellum in the cis-eyespot flagellum. Electron microscopic studies suggest that the developmental defect resides in the basal body. Extensive recombination analysis to 33 nuclear markers representing the 16 linkage groups failed to establish linkage. The uni mutants, however, showed linkage to four unmapped mutant loci. Mutations for each of these loci also affect flagellar assembly.