RELEASE OF ENDOGENOUS 5-HYDROXYTRYPTAMINE FROM RESTING AND STIMULATED ENTERIC NEURONS
RELEASE OF ENDOGENOUS 5-HYDROXYTRYPTAMINE FROM RESTING AND STIMULATED ENTERIC NEURONS
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DOI：
10.1016/0306-4522(81)90017-8
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发表时间：
1981-01-01
期刊：
影响因子：
3.3
通讯作者：
TAMIR, H
中科院分区：
文献类型：
--
作者：
GERSHON, MD;TAMIR, H
本文报道了肠神经元内源性5-羟色胺的释放。将豚鼠小肠段翻转并通过新创建的浆膜腔进行体外灌注。[3 H]5-羟色胺试验显示存在组织屏障，阻止粘膜（肠内分泌细胞）5-羟色胺扩散到灌注液中;灌注液中的所有5-羟色胺均为神经源性。肠道受到电刺激。5-灌注液和肌间神经丛中的羟色胺通过特异性放射酶法测定。5-羟色胺存在于肌间神经丛;它被释放到灌注液自发和释放增强电刺激。刺激，但不是自发的，释放的胺是Ca 2+依赖。与新摄取的[3 H]5-羟色胺的释放比较表明，电释放的5-羟色胺的比放射性高于自发释放的或组织胺的比放射性。刺激也增加了5-羟色胺的释放超过其代谢产物在灌注液中。5-羟色胺显然是肠神经系统的内源性成分，其通过肠神经的电场刺激而释放。新采取的5-羟色胺可能是由这些神经元优先释放。
The release of endogenous 5-hydroxytryptamine from enteric neurons is reported. Segments of guinea-pig small intestine were everted and perfused in vitro through the newly created serosal lumen. Tests with [3H]5-hydroxytryptamine revealed the existence of a tissue barrier preventing diffusion of mucosal (enteroendocrine cell) 5-hydroxytryptamine into the perfusate; all 5-hydroxytryptamine in the perfusate was of neural origin. The gut was stimulated electrically. 5-Hydroxytryptamine in the perfusate and in the myenteric plexus was assayed by a specific radioenzymatic method. 5-Hydroxytryptamine was present in the myenteric plexus; it was released into the perfusate spontaneously and the release was enhanced by electrical stimulation. The stimulated, but not the spontaneous, release of the amine was Ca2+-dependent. Comparison with the release of newly taken up [3H]5-hydroxytryptamine showed that the specific radioactivity of electrically released 5-hydroxytryptamine was higher than that of either the spontaneously released or tissue amine. Stimulation also increased the release of 5-hydroxytryptamine more than that of its metabolites in the perfusate. 5-Hydroxytryptamine is apparently an endogeneous constituent of the enteric nervous system which is released by electrical field stimulation of enteric nerves. Newly taken up 5-hydroxytryptamine is probably released preferentially by these neurons.