Comparative evaluation of chitosan-based multifunctional wound dressings: a multistage randomised controlled experimental trial
Comparative evaluation of chitosan-based multifunctional wound dressings: a multistage randomised controlled experimental trial
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DOI：
10.25207/1608-6228-2021-28-3-78-96
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发表时间：
2021-01-01
期刊：
影响因子：
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通讯作者：
Grigoriev, T. Е.
中科院分区：
文献类型：
--
作者：
Ushmarov, D. I.;Gumenyuk, S. E.;Grigoriev, T. Е.
背景。各种病因造成的伤口是最常见的外伤之一。改善这种疾病治疗的一个前瞻性途径是开发新颖的或改进已经在舞台上的敷料材料。 目标。使用超声波控制所开发剂型的生物降解，对新型基于壳聚糖的伤口敷料在不同成因的软组织伤口愈合中进行比较实验测定。方法。使用专有方法（2018 年 8 月 23 日发明专利 RU No. 2703709）在实验动物、体重 275 (± 25) g 的传统和血统雄性大鼠和体重 2,900 (± 150) g 的雄性兔子中建立软组织伤口模型。在最后一步中测试了被选为最有希望且适用于大体积软组织无菌伤口的两种伤口敷料样品。实验。在样品材料生物降解过程中对模型伤口区域进行超声成像，以更详细地控制伤口过程。在实验的所有步骤中，动物模型的处理均符合 GOST 33044-2014“良好实验室规范原则”。组织形态学测定按照通用方案进行。数据分析采用变异统计，包括均值（M）和标准均值误差（± m）估计。统计显着性水平为 p < 0.05。结果。动物试验和对所获得数据的综合评估表明，基于壳聚糖的伤口涂层具有很高的愈合功效。伤口敷料样品的分析证实了它们预先设计的特性：支架稳定性、毛细管作用、生物降解性和所载药物输送的基质能力。此外，超声波被证明可以有效估计涂层伤口愈合和生物聚合物生物降解过程的动态。结论。所开发的基于壳聚糖的伤口敷料在软组织伤口过程的实验模型中表现出功效。使用超声波研究伤口过程的动力学和涂层生物降解提供了成像和组织组织形态学数据以及涂层材料的结构和性能之间的充分一致性。
Background. Wounds of various aetiology are among the most frequent traumatic injuries. A prospective route to improve treatment of this nosology is the development of novel or advancement of the already on-stage dressing materials.Objectives. A comparative experimental assay of novel chitosan-based wound dressings in the healing of soft tissue wounds of different genesis using ultrasound to control biodegradation of the developed dosage form.Methods. Soft tissue wounds were modelled in experimental animals, conventional and lineage male rats, 275 (± 25) g body weight, and male rabbits, 2,900 (± 150) g weight, using a proprietary methodology (Patent on invention RU No. 2703709 dated 23 August, 2018).Two wound dressing samples selected as most promising and applicable in large-volume soft tissue aseptic wounds were tested in the final step of experiment. Ultrasound imaging of the model wound area during the sample material biodegradation was used to control the wound process in more detail. At all steps of experiment, animal models were handled in compliance with GOST 33044-2014 “Principles of Good Laboratory Practice”.Histomorphological assays were carried out with common protocols. Variation statistics was used for the data analysis, including the mean (M) and standard mean error (± m) estimation. The level of statistical significance was p < 0.05.Results. The animal assays and comprehensive evaluation of the obtained data revealed a high healing efficacy of chitosan-based wound coatings. The analyses of wound dressing samples have confirmed their predesigned properties: scaffold stability, capillarity, biodegradability and matrix capacities for the carried drug delivery.Additionally, ultrasound was proved effective to estimate dynamics of the coated wound healing and biopolymer biodegradation processes.Conclusion. The developed chitosan-based wound dressings exhibited efficacy in an experimental model of the soft tissues wound process.The use of ultrasound to study dynamics of the wound process and coating biodegradation provided an adequate congruence between the imaging and tissue histomorphology data, as well as the structure and properties of coating material.