Bright Near-Infrared p-Conjugated Oligomer Nanoparticles for Deep-Brain Three-Photon Microscopy Excited at the 1700 nm Window in Vivo
Bright Near-Infrared p-Conjugated Oligomer Nanoparticles for Deep-Brain Three-Photon Microscopy Excited at the 1700 nm Window in Vivo
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1700 nm窗口激发的明亮近红外p-共轭低聚物纳米粒子用于脑深部三光子显微镜
DOI：
10.1021/acsnano.2c03813
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发表时间：
2022-08-15
期刊：
影响因子：
17.1
通讯作者：
Lee, Chun-Sing
中科院分区：
文献类型：
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作者：
Li, Shengliang;Deng, Xiangquan;Lee, Chun-Sing
具有1700 nm激发的三光子荧光团的发展对于位于脑深处的组织的体内成像是迫切需要的。在此，我们报告了一种设计和合成的荧光分子（OFET）在体内小鼠脑成像与三光子显微镜在记录成像深度。OFET分子具有相对高的荧光亮度，并且在整合为水分散性纳米颗粒（OEFT NP）后在820 nm处具有近红外（NIR）最大发射。在1720 nm激发下，OFET NP显示出1.06 x 10（-82）cm（6）s（2）/光子（2）的大的三光子作用截面，这是常用的磺酰罗丹明101（SR 101）染料的两倍多。受益于1720 nm的第二NIR窗口中的长激发和820 nm的第一NIR窗口中的发射波长的高组织穿透深度、高亮度和三光子的大作用截面，OFET NP具有良好的脑深部成像性能。位于1696 μ m深度的小鼠脑血管在体内可以清楚地分辨。由于即使在高浓度下也没有可观察到的细胞毒性，本发明的OFET纳米颗粒表明荧光缀合的寡聚体在体内深部组织的高分辨率3 PM成像中具有巨大的潜力。
The development of three-photon fluorophores with 1700 nm excitation is pressingly desirable for in vivo imaging of tissue resided deep inside the brain. Herein, we report a designed and synthesized fluorescent molecule (OFET) for in vivo mouse brain imaging with three-photon microscopy at a record imaging depth. The OFET molecule has a relatively high fluorescence brightness and has a near-infrared (NIR) maximum emission at 820 nm after integrating as water dispersible nanoparticles (OEFT NPs). Under 1720 nm excitation, OFET NPs show a large three-photon action crosssection of 1.06 x 10(-82) cm(6) s(2)/photon(2), which is more than twice that of the commonly used sulforhodamine 101 (SR101) dye. Benefiting from the high tissue penetration depths for both the long excitation in the second NIR window of 1720 nm and the emission wavelength in the first NIR window of 820 nm, a high brightness, and a large action cross-section of three-photon, OFET NPs have good deep-brain imaging performance. Brain vasculatures of a mouse located at a depth of 1696 mu m can be clearly resolved in vivo. With no observable cytotoxicity even in a high concentration, the present OFET NPs suggest that fluorescent it-conjugated oligomers are of great potential in high-resolution 3PM imaging of in vivo deep-tissue.