Aggregation-induced emission (AIE)-active fluorescent probes with multiple binding sites toward ATP sensing and live cell imaging
Aggregation-induced emission (AIE)-active fluorescent probes with multiple binding sites toward ATP sensing and live cell imaging
复制标题
具有多个结合位点的聚集诱导发射 (AIE) 活性荧光探针，可用于 ATP 传感和活细胞成像
DOI：
10.1039/c7tb02399e
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发表时间：
2017
影响因子：
7
通讯作者：
杨媛
中科院分区：
文献类型：
--
作者：
马恒昌;杨满义;张彩丽;马钰程;秦艳芳;雷自强;常璐;雷蕾;王涛;杨媛
聚集诱导发射（AIE）活性化合物是一种有吸引力的荧光材料，应用于化学和生物传感。这类材料的AIE效应放大了由聚集到分解的物理状态转变导致的荧光信号变化，可用于检测各种分析物，灵敏度高。特别是，特定生物活性分析物的识别不仅非常有趣，而且具有挑战性。在本文中，我们报道了一组新的含有吡啶和硼酸基团（TPA-PP, TPA-PPA-1, TPA-PPA-2, TPA-PPA-3）的aie活性荧光探针，它们已被开发用于识别腺苷5 ' -三磷酸（ATP）。这些探针具有两种相互作用模式和与ATP分子的多个连接位点，能够选择性地区分ATP和其他生物活性阴离子，并显著增强荧光发射。特别是在细胞成像的应用中，随着正电荷和硼酸基团数量的进一步增加，探针可以穿透细胞，然后非常特异性地进入细胞核。这些结果清楚地表明，新开发的传感器适用于不同细胞器的特异性跟踪，具有高度可视化和保留能力。因此，它们都被证实是未来活细胞成像的有希望的替代方案。
Aggregation-induced emission (AIE)-active compounds are attractive fluorescent materials for applications in chemical and biological sensing. The AIE effect of such materials amplifies changes in the fluorescence signal due to the physical state transformation from aggregation to disaggregation, which can be employed for detecting various analytes with high sensitivity. In particular, specific bio-active analyte recognition is not only very interesting but also challenging. In this paper, we report a set of novel AIE-active fluorescent probes containing pyridiniums and boric acid groups (TPA-PP, TPA-PPA-1, TPA-PPA-2, TPA-PPA-3), which has been developed for adenosine 5′-triphosphate (ATP) recognition. These probes with two types of interaction modes and multiple connection sites toward ATP molecules are able to selectively discriminate ATP among other bioactive anions with a significant enhancement in fluorescence emission. In particular, in the application of cell imaging, as the number of positive charges and boric acid group increased further, the probes could penetrate into cells, and then enter into the nucleus very specifically. These results clearly demonstrate that the newly developed sensors are suitable for specific tracing of different cell organelles with a height visualization and retention ability. Therefore, all of them are confirmed as promising alternatives for live cell imaging in the future.