An increase in the energy coupling capacity of submitochondrial particles in the presence of lanthanides.
An increase in the energy coupling capacity of submitochondrial particles in the presence of lanthanides.
复制标题
在镧系元素存在下，亚软骨粒子的能量耦合能力增加。
DOI：
10.1016/0014-5793(94)00711-x
复制
发表时间：
1994
期刊：
影响因子：
3.5
通讯作者：
Vinogradov,AD
中科院分区：
文献类型：
--
作者：
Grivennikova,VG;Gavrikova,EV;Vinogradov,AD
用过量寡霉素处理的由内而外的亚线粒体颗粒的NADH和琥珀酸氧化酶活性被镧系元素（La 3+和Dy 3+）抑制。寡霉素和寡霉素加镧系元素的抑制作用完全解除解偶联剂。因此，在镧系元素存在下，呼吸控制（测量为向寡霉素处理的颗粒中添加解偶联剂引起的NADH或琥珀酸氧化的刺激）增加。镧系元素的偶联作用被过量的EDTA完全阻止并迅速逆转。La3+增加了寡霉素处理的亚线粒体颗粒催化的有氧能量连接的琥珀酸盐支持的NAD+还原的程度。镧系元素似乎是一个有用的工具，以增加亚线粒体颗粒的能量耦合能力。
NADH and succinate oxidase activities of inside‐out submitochondrial particles treated with excess oligomycin are inhibited by lanthanides (La3+and Dy3+). Inhibition by both oligomycin and oligomycin plus lanthanides is completely relieved by an uncoupler. The respiratory control, measured as the stimulation of NADH or succinate oxidation caused by the addition of uncoupler to the oligomycin‐treated particles, is thus increased in the presence of lanthanides. The coupling effect of lanthanides is completely prevented and rapidly reversed by excess of EDTA. La3+increases the extent of the aerobic energy‐linked succinate‐supported NAD+reduction catalyzed by the oligomycin‐treated submitochondrial particles. Lanthanides seem to be a useful tool to increase the energy coupling capacity of the submitochondrial particles.