Infrared-based module for the synthesis of 68Ga-labeled radiotracers
Infrared-based module for the synthesis of 68Ga-labeled radiotracers
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DOI：
10.1016/j.nucmedbio.2006.10.011
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发表时间：
2007-01-01
影响因子：
3.1
通讯作者：
Mourtada, Firas
中科院分区：
文献类型：
--
作者：
Azhdarinia, Ali;Yang, David J.;Mourtada, Firas
简介：发生器产生的正电子发射断层扫描示踪剂最近得到了很大的关注，由于良好的成像特性。可获得性和可负担性。本研究的重点是设计和验证一个半自动化的Ga-68标记的化学模块，利用红外线为基础的加热快速控制的热cycle.Methods：原型模块建立并安装在我们的实验室。DOTA（1，4，7，10-四氮杂环十二烷-1，4，7，10-四乙酸）用Ga-68手动标记（10-1000 nmol）以优化合成条件。为了自动化，用Ga-68标记250 nmol DOTA，反应时间为5 min（n =5）、10 min（n =5）和20 min（n =6）。一个剂量校准器和放射性薄层色谱法被用来访问手动和自动synthes.Results的产品产量和质量：开发了一个半自动68 Ga合成模块。该系统表明，软件控制可用于驱动多步放射化学合成，并可生产放射化学纯度> 95%的Ga-68-DOTA，类似于手动合成。该装置还表明，对于5分钟的短反应时间，可以实现> 70%的衰变校正放射性产率。整个合成过程仅需22 min，其中6-8 min用于HCl蒸发。该过程的温度和压力曲线是一致的。结论：我们证明了使用市售的Ga-68/Ge-68发生器与半自动化模块成功地标记的双功能螯合剂DOTA与Ga-68。不同的Ga-68标记的生物共轭物的进一步调查是必要的，以证明该模块作为示踪剂开发和成像研究的工具的有用性。(c)2007年爱思唯尔公司All rights reserved.
Introduction: Generator-produced positron emission tomography tracers have gained much attention recently due to favorable imaging characteristics. accessibility and affordability. The focus of this study was to design and validate a semiautomated module for Ga-68-labeled chemistry utilizing infrared-based heating for rapid control of thermal cycle.Methods: A prototype module was built and installed in our laboratory. DOTA (1,4,7,10-tetra-azacyclododecane-1,4,7,10-tetra-acetic acid) was manually labeled (10-1000 nmol) with Ga-68 to optimize synthesis conditions. For automation, 250 nmol of DOTA was labeled with Ga-68 with reaction times of 5 min (n =5), 10 min (n =5) and 20 min (n =6). A dose calibrator and radio-thin-layer chromatography were used to access the product yield and quality of both manual and automated syntheses.Results: A semiautomated 68Ga synthesis module was developed. The system showed that software control could be used to drive a multistep radiochemical synthesis and to produce Ga-68-DOTA with > 95% radiochemical purity, similar to that in manual synthesis. The device also showed that for a short reaction time of 5 min, decay-corrected radioactive yields of > 70% could be achieved. The total synthesis was as short as 22 min, including 6-8 min for HCl evaporation. The temperature and pressure profiles of the process were consistent.Conclusion: We demonstrated the use of a commercially available Ga-68/Ge-68 generator with a semiautomated module to successfully label the bifunctional chelator DOTA with Ga-68. Further investigation with different Ga-68-labeled bioconjugates is warranted to demonstrate the usefulness of the module as a tool for tracer development and imaging research. (c) 2007 Elsevier Inc. All rights reserved.