Improved Muscle Regeneration into a Joint Prosthesis with Mechano-Growth Factor Loaded within Mesoporous Silica Combined with Carbon Nanotubes on a Porous Titanium Alloy.
Improved Muscle Regeneration into a Joint Prosthesis with Mechano-Growth Factor Loaded within Mesoporous Silica Combined with Carbon Nanotubes on a Porous Titanium Alloy.
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DOI：
10.1021/acsnano.2c04591
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发表时间：
2022-09
期刊：
影响因子：
17.1
通讯作者：
Xiaoqin Wei;Qin Chen;L. Bu;Xi Wan;Z. Jiao;Zixiang Han;D. Zou;Jisi Zheng;Chi Yang
中科院分区：
文献类型：
--
作者：
Xiaoqin Wei;Qin Chen;L. Bu;Xi Wan;Z. Jiao;Zixiang Han;D. Zou;Jisi Zheng;Chi Yang
全关节置换术（TJR）被广泛应用于严重关节疾病的重建，但通常以骨骼肌与金属关节假体的附着严重丧失为特征，导致纤维瘢痕组织形成和随后的运动功能障碍。组织工程技术可能为骨骼肌再生为金属关节假体提供一种潜在的策略。在这里，多孔钛（Ti）合金支架与碳纳米管（CNTs）和介孔二氧化硅纳米粒子（MSNs）通过电泳沉积（EPD）被设计为机械生长因子（MGF）的载体。这种双层涂层具有纳米结构拓扑结构、优异的MGF负载和通过共价键合的延长释放性能，以改善多孔钛合金支架中的成肌细胞粘附、增殖和肌原分化，而无细胞毒性。Akt/mTOR信号通路在这一过程中起着关键作用。此外，体内研究表明，支架促进肌肉（而不是纤维化组织）生长到多孔钛合金结构中，并改善肌肉衍生的机械性能、卫星细胞的迁移以及可能的免疫调节。总之，这种纳米材料涂层支架提供了一个实用的生物材料平台，可以再生假体周围的肌肉组织，并恢复与天然关节相当的运动功能。
Total joint replacement (TJR) is widely applied as a promising treatment for the reconstruction of serious joint diseases but is usually characterized by critical loss of skeletal muscle attachment to metal joint prostheses, resulting in fibrous scar tissue formation and subsequent motor dysfunction. Tissue engineering technology may provide a potential strategy for skeletal muscle regeneration into metal joint prostheses. Here, a porous titanium (Ti) alloy scaffold coated with carbon nanotubes (CNTs) and mesoporous silica nanoparticles (MSNs) through electrophoretic deposition (EPD) was designed as a mechano-growth factor (MGF) carrier. This two-layered coating exhibits a nanostructured topology, excellent MGF loading, and prolonged release performance via covalent bonding to improve myoblast adhesion, proliferation and myogenic differentiation in porous Ti alloy scaffolds without cytotoxicity. The Akt/mTOR signaling pathway plays a key role in this process. Furthermore, in vivo studies show that the scaffold promotes the growth of muscle, rather than fibrotic tissue, into the porous Ti alloy structure and improves muscle-derived mechanical properties, the migration of satellite cells, and possibly immunomodulation. In summary, this nanomaterial-coated scaffold provides a practical biomaterial platform to regenerate periprosthetic muscle tissue and restore comparable motor function to that of the natural joint.