Sequence of the 20-kilodalton heavy chain peptide from the carboxyl-terminus of bovine cardiac myosin subfragment-1.
Sequence of the 20-kilodalton heavy chain peptide from the carboxyl-terminus of bovine cardiac myosin subfragment-1.
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来自牛心肌肌球蛋白亚片段 1 羧基末端的 20 千道尔顿重链肽的序列。
DOI：
10.1172/jci111462
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Morkin,E
中科院分区：
文献类型：
--
作者：
Flink,IL;Morkin,E
通过自动顺序降解未消化的肽和化学和酶消化衍生的亚片段，确定了来自牛心脏肌球蛋白亚片段-1（S-1）的羧基末端20 kD胰蛋白酶重链肽的几乎完整的氨基酸序列。该片段含有169个残基，包括两个反应性半胱氨酰残基，它们相隔9个残基。在序列中的6个位置，存在两个氨基酸残基，并分离出两种不同版本的胰凝乳蛋白酶肽，产率约为53%和24%，表明该物种中存在两种心肌肌球蛋白β型重链。20-kD肽的二级结构分析预测片段内有两个不同的区域。第一区域（残基1-121）含有12%的α-螺旋、25%的β-折叠、40%的β-弯曲和19%的卷曲;第二区域（残基122-169）可以形成延伸的α-螺旋。牛序列与含有编码兔心肌肌球蛋白β-重链（pMHC β 174）的DNA序列的重组质粒的推导氨基酸序列的比较显示约86%的同源性。
An almost complete amino acid sequence of the carboxyl-terminal 20-kD tryptic heavy chain peptide from bovine cardiac myosin Subfragment-1 (S-1) has been determined by automated sequential degradation of the undigested peptide and subfragments derived by chemical and enzymatic digestion. The fragment contains 169 residues, including two reactive cysteinyl residues which are located nine residues apart. At six positions in the sequence, two amino acid residues were present and two different versions of a chymotryptic peptide were isolated in approximately 53 and 24% yields, suggesting that there are two cardiac myosin beta-type heavy chains in this species. Analysis of the secondary structure of the 20-kD peptide predicts that there are two distinct regions within the fragment. The first region (residues 1-121) contains 12% alpha-helix, 25% beta-sheet, 40% beta-bends, and 19% coil; the second region (residues 122-169) may form an extended alpha-helix. Comparison of the bovine sequence with the deduced amino acid sequence of a recombinant plasmid containing DNA sequences coding for the beta-heavy chain of rabbit cardiac myosin (pMHC beta 174) reveals approximately 86% homology.Images