Utilizing pyrene-degrading endophytic bacteria to reduce the risk of plant pyrene contamination
Utilizing pyrene-degrading endophytic bacteria to reduce the risk of plant pyrene contamination
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DOI：
10.1007/s11104-013-1875-x
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发表时间：
2013
期刊：
影响因子：
4.9
通讯作者：
Kai Sun;Juan Liu;Li-hua Jin;Yanzheng Gao
中科院分区：
文献类型：
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作者：
Kai Sun;Juan Liu;Li-hua Jin;Yanzheng Gao
目的内生细菌普遍存在于植物体内，但内生细菌对植物吸收和代谢多环芳烃的影响研究较少。因此，我们试图研究降解多环芳烃的内生细菌在目标植物上的定殖是否会改善植物的多环芳烃代谢，降低植物多环芳烃污染的风险。方法通过浓缩培养从多环芳烃污染的植物中分离到一株能降解芘的内生菌。将分离的细菌接种到根部后，进行温室容器试验。采用高效毛细管电泳法对土壤和植物样品中的芘残留量进行分析。BJ06是从赤豆杉中分离到的一株能在15d内降解56.0%的芘(50 mg · L−1)的菌株。BJ06菌株在环境条件下能高效生长并降解芘。在容器试验中，该菌显著促进了黑麦草的生长和对污染土壤中芘的去除。接种内生菌的黑麦草根部和地上部中的芘浓度分别比未接种内生菌的土壤降低了31.01%和44.22%。结论为利用内生细菌调控植物吸收有机污染物提供了新的视角。这一研究结果将对植物多环芳烃污染的风险评估具有参考价值。
AimsEndophytic bacteria are ubiquitous in plants, but little information is available on the influence of endophytic bacteria on the uptake and metabolism of PAH by plants. Thus, we seek to investigate whether the colonization of a target plant by a PAH-degrading endophytic bacterium would improve the PAH metabolism of the plant and reduce the risk of plant PAH contamination.MethodsA pyrene-degrading endophyte was isolated from PAH-contaminated plants using enrichment culture. After root inoculation with the isolated bacterium, greenhouse container experiments were conducted. Pyrene residues in soil and plant samples were analyzed by HPLC.ResultsA pyrene-degrading endophytic bacterium,Staphylococcussp. BJ06, was isolated fromAlopecurus aequalisand could degrade 56.0 % of pyrene (50 mg · L−1) within 15 days. BJ06 grew and degraded pyrene efficiently under environmental conditions. The bacterium significantly promoted ryegrass growth and pyrene removal from contaminated soil in container experiments. The pyrene concentrations in ryegrass roots and shoots in endophyte-inoculated planted soil were reduced by 31.01 % and 44.22 %, respectively, compared with endophyte-free planted soil.ConclusionsWe have provided new perspectives on the regulation and control of plant uptake of organic contaminants with endophytic bacteria. The results of this study will be valuable to risk assessments of plant PAH contamination.