Interaction of bacteriophage T4 AsiA protein with Escherichia coli σ 70 and its variant
Interaction of bacteriophage T4 AsiA protein with Escherichia coli σ
70 and its variant
复制标题
噬菌体 T4 AsiA 蛋白与大肠杆菌的相互作用 σ
DOI：
10.1016/s0014-5793(97)00668-6
复制
发表时间：
1997
期刊：
影响因子：
3.5
通讯作者：
D. Chatterji
中科院分区：
文献类型：
--
作者：
S. Pahari;D. Chatterji
T4噬菌体产生一种小蛋白AsiA，该蛋白通过与σ70紧密结合抑制大肠杆菌中σ70依赖性启动子的转录，因此被称为抗σ因子。我们观察到，在RNA聚合酶和模板DNA之间预先形成的开放复合物中加入AsiA对lac UV5启动子的单轮转录没有抑制作用。然而，在AsiA存在的情况下，转录在“扩展- 10”启动子上正常进行。因此，亚洲在其4.2子域或其附近结合了σ70。进一步对σ70和突变体σ70-V576G的免疫沉淀实验似乎证实了这一结论。
Bacteriophage T4 produces a small protein AsiA, which inhibits transcription from σ70-dependent promoters in E. coli by tightly binding to σ70and is therefore termed as anti-sigma factor. We observed that there was no inhibition of single round transcription at lac UV5 promoter when AsiA was added to preformed open complex between RNA polymerase and template DNA. However, transcription was found to proceed normally at `extended −10' promoters in the presence of AsiA. It appears therefore that AsiA binds σ70at its 4.2-subdomain or in its close vicinity. Further experiments on immunoprecipitation of σ70and a mutant σ70-V576G with AsiA seem to corroborate such conclusion.
影响因子：
5.6
作者：
SIEGELE, DA;HU, JC;GROSS, CA
通讯作者：
GROSS, CA
影响因子：
5.6
作者：
GARDELLA, T;MOYLE, H;SUSSKIND, MM
通讯作者：
SUSSKIND, MM