DNA barcoding as a heuristic tool for classifying undescribed Nearctic Myrmica ants (Hymenoptera: Formicidae)
DNA barcoding as a heuristic tool for classifying undescribed Nearctic Myrmica ants (Hymenoptera: Formicidae)
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DOI：
10.1111/j.1463-6409.2009.00386.x
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发表时间：
2009-09-01
影响因子：
2.5
通讯作者：
Vepsalainen, Kari
中科院分区：
文献类型：
--
作者：
Jansen, Gunther;Savolainen, Riitta;Vepsalainen, Kari
古北界蚁属Myrmica的种类研究得很好。相比之下，新北种的分类学已经过时，无法识别。我们收集了桃金娘样本在泛北极和调查其多样性使用mtDNA数据。我们分析了57古北和293新北Myrmica样品的细胞色素氧化酶I基因的条形码序列。我们使用已知的古北物种的序列来搜索Myrmica条形码模式。除一个古北种组外，所有古北种组均已恢复。新北区的多样性比已知的要高得多。我们检索punctiventris，crassirugis和不完全组，并建立了9个额外的暂定种组。遗传距离分析显示，古北种和种组的种内和种间距离有很大的重叠。我们不能找到一个变异的差距，分离新北序列的物种与COI数据。花葶形态变异进一步划分为两个遗传类群。花葶形态与大多数分子组，除了三个标本。我们的研究结果表明，条形码，只使用有限的遗传信息，不能作为一个通用的代理分类和物种划分。这应该被视为了解一个未知生物群体的分类多样性的第一步。
The Palearctic species of the ant genus Myrmica are well studied. In contrast, the taxonomy of the Nearctic species is outdated, making identification impossible. We collected Myrmica samples in the Holarctic and investigated their diversity using mtDNA data. We analysed a barcode sequence of the Cytochrome Oxidase I gene for 57 Palearctic and 293 Nearctic Myrmica samples. We used sequences of known Palearctic species to search for Myrmica barcode patterns. All but one Palearctic species groups were recovered. The Nearctic diversity was much higher than known. We retrieved the punctiventris, crassirugis and incompleta groups, and established nine additional tentative species groups. Genetic distance analysis revealed a large overlap of intra- and inter-specific distances in Palearctic species and species groups. We could not find a variation gap to separate Nearctic sequences into species with COI data only. Variation in scape morphology divided two genetic groups further. Scape morphology correlated with most molecular groups, except three specimens. Our results illustrate that barcoding, using only a limited amount of genetic information, cannot serve as a universal proxy for taxonomy and species demarcation. It should be considered a first step in understanding the taxonomic diversity of an unknown group of organisms.