Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells.
Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells.
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DOI：
10.5395/rde.2012.37.3.142
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发表时间：
2012-08
期刊：
影响因子：
--
通讯作者：
Baek SH
中科院分区：
文献类型：
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作者：
Seo MS;Hwang KG;Kim H;Baek SH
我们分析了基因表达谱后14天的人牙髓细胞（DPC）牙诱导使用DNA微阵列，并寻找可能与矿化相关的候选基因。将人牙髓细胞在牙源性诱导培养基（OM）中培养14天，获得诱导后的人牙髓细胞。暴露于正常培养基的细胞用作对照。从细胞中提取总RNA并通过微阵列分析进行分析，并通过逆转录聚合酶链反应（RT-PCR）选择性地确认关键结果。我们还进行了基因集富集分析（GSEA）的微阵列数据。珠芯片上的47,320个探针中有605个基因在诱导细胞中的差异超过两倍。其中，217个基因表达上调，388个基因表达下调。GSEA显示，在诱导的细胞中，涉及无翅MMTV整合的细胞凋亡和信号转导（Wnt）的基因显著上调。Wnt介导的凋亡和信号转导基因与牙髓细胞向成牙本质细胞的分化密切相关。
We analyzed gene-expression profiles after 14 day odontogenic induction of human dental pulp cells (DPCs) using a DNA microarray and sought candidate genes possibly associated with mineralization. Induced human dental pulp cells were obtained by culturing DPCs in odontogenic induction medium (OM) for 14 day. Cells exposed to normal culture medium were used as controls. Total RNA was extracted from cells and analyzed by microarray analysis and the key results were confirmed selectively by reverse-transcriptase polymerase chain reaction (RT-PCR). We also performed a gene set enrichment analysis (GSEA) of the microarray data. Six hundred and five genes among the 47,320 probes on the BeadChip differed by a factor of more than two-fold in the induced cells. Of these, 217 genes were upregulated, and 388 were down-regulated. GSEA revealed that in the induced cells, genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) were significantly upregulated. Genes implicated in Apoptosis and Signaling by Wnt are highly connected to the differentiation of dental pulp cells into odontoblast.