Metal Nanoparticle Modified Carbon-Fiber Microelectrodes Enhance Adenosine Triphosphate Surface Interactions with Fast-Scan Cyclic Voltammetry.
Metal Nanoparticle Modified Carbon-Fiber Microelectrodes Enhance Adenosine Triphosphate Surface Interactions with Fast-Scan Cyclic Voltammetry.
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金属纳米颗粒修饰碳纤维微电极增强三磷酸腺苷表面相互作用的快速扫描循环伏安法。
DOI：
10.1021/acsmeasuresciau.1c00026
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发表时间：
2022-04-20
期刊：
影响因子：
--
通讯作者：
Ross, Ashley E
中科院分区：
文献类型：
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作者：
Li, Yuxin;Keller, Alexandra L;Cryan, Michael T;Ross, Ashley E
三磷酸腺苷（ATP）是一种重要的快速信号分子，参与机体的多种病理过程。从历史上看，ATP是很难直接检测电化学与快速扫描循环伏安法（FSCV）由于有限的相互作用在裸露的碳纤维。ATP如何在电极表面相互作用的系统研究是必要的开发更灵敏的电化学检测方法。在这里，我们开发了金纳米颗粒（AuNP）和铂纳米颗粒（PtNP）修饰的碳纤维微电极耦合到FSCV来测量ATP在金属纳米颗粒修饰的表面相互作用的程度，并提高直接电化学检测的灵敏度。通过在0.5 mg/mL HAuCl 4或0.5 mg/mLK 2 PtCl 6中从-1.2至1.5 V扫描30 s，将AuNP和PtNP电沉积在碳纤维表面上。总的来说，我们证明了在AuNP修饰的电极上氧化ATP电流平均增加4.1 ± 1.0倍，在PtNP修饰的电极上增加3.5 ± 0.3倍。金属纳米颗粒改性的表面促进了ATP氧化产物在表面的电催化转化，促进了增强的吸附强度和表面覆盖度，并显着提高了灵敏度。在外源性应用后，在活的小鼠淋巴结组织内成功地检测到ATP。总的来说，这项研究证明了金属纳米颗粒表面ATP氧化的详细表征和组织中ATP直接电化学检测的显着改进的方法。
Adenosine triphosphate (ATP) is an important rapid signaling molecule involved in a host of pathologies in the body. Historically, ATP is difficult to directly detect electrochemically with fast-scan cyclic voltammetry (FSCV) due to limited interactions at bare carbon-fibers. Systematic investigations of how ATP interacts at electrode surfaces is necessary for developing more sensitive electrochemical detection methods. Here, we have developed gold nanoparticle (AuNP), and platinum nanoparticle (PtNP) modified carbon-fiber microelectrodes coupled to FSCV to measure the extent to which ATP interacts at metal nanoparticle-modified surfaces and to improve the sensitivity of direct electrochemical detection. AuNP and PtNPs were electrodeposited on the carbon-fiber surface by scanning from −1.2 to 1.5 V for 30 s in 0.5 mg/mL HAuCl4 or 0.5 mg/mLK2PtCl6. Overall, we demonstrate an average 4.1 ± 1.0-fold increase in oxidative ATP current at AuNP-modified and a 3.5 ± 0.3-fold increase at PtNP-modified electrodes. Metal nanoparticle-modified surfaces promoted improved electrocatalytic conversion of ATP oxidation products at the surface, facilitated enhanced adsorption strength and surface coverage, and significantly improved sensitivity. ATP was successfully detected within living murine lymph node tissue following exogenous application. Overall, this study demonstrates a detailed characterization of ATP oxidation at metal nanoparticle surfaces and a significantly improved method for direct electrochemical detection of ATP in tissue.