Methanolobus profundi sp nov., a methylotrophic methanogen isolated from deep subsurface sediments in a natural gas field
Methanolobus profundi sp nov., a methylotrophic methanogen isolated from deep subsurface sediments in a natural gas field
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DOI：
10.1099/ijs.0.001677-0
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发表时间：
2009-04-01
影响因子：
2.8
通讯作者：
Kamagata, Yoichi
中科院分区：
文献类型：
--
作者：
Mochimaru, Hanako;Tamaki, Hideyuki;Kamagata, Yoichi
从日本天然气田分离到一株嗜中温甲基营养型产甲烷菌MobM（T）。菌株MobM（T）在甲醇和甲胺上生长，但在H-2/CO2、甲酸盐、乙酸盐或二甲基硫醚上不生长。这些细胞是能动的、不规则的球菌（直径0.9-1.2 μ m），单个、成对、四球菌或（偶尔）聚集在一起。菌株MobM（T）在9-37 ℃（最佳在30 ℃）和pH 6.1-7.8（最佳在pH 6.5）下生长。生长需要钠和镁，0.1-1.0 M Na+（最佳为0.35 M）和10-400 MM Mg 2+（最佳为15-25 mM）。基因组DNA的G+C含量为42.4mol%。16 S rRNA基因测序显示，该分离物是Methanolobus属的成员，但与其最近的邻居Methanolobus tindarius DSM 2278（T）（序列相似性为98.0%）和Methanolobus vulcani DSM 3029（T）（98.1%）不同。基于MobM（T）的表型和系统发育特征，很明显，该菌株代表了Methanolobus属的一个新物种，因此建议将其命名为Methanolobus profundi sp. nov.。模式菌株是MobM（T）（=DSM 21213（T）=NBRC 104158（T））。
A mesophilic, methylotrophic methanogen, strain MobM(T), was isolated from a natural gas field in Japan. Strain MobM(T) grew on methanol and methylamines, but not on H-2/CO2, formate, acetate or dimethyl sulfide. The cells were motile, irregular cocci (diameter, 0.9-1.2 mu m) and occurred ;singly, in pairs, as tetracocci or (occasionally) as aggregates. Strain MobM(T) grew at 9-37 degrees C (optimally at 30 degrees C) and at pH 6.1-7.8 (optimally at pH 6.5). Sodium and magnesium were required for growth, at 0.1-1.0 M Na+ (optimally at 0.35 M) and 10-400 MM Mg2+ (optimally at 15-25 mM). The G+C content of the genomic DNA was 42.4 mol%. 16S rRNA gene sequencing revealed that the isolate is a member of the genus Methanolobus, but distinct from its closest neighbours, Methanolobus tindarius DSM 2278(T) (sequence similarity, 98.0%) and Methanolobus vulcani DSM 3029(T) (98.1%). On the basis of phenotypic and phylogenetic features of MobM(T), it is clear that this strain represents a novel species of the genus Methanolobus, for which the name Methanolobus profundi sp. nov. is proposed. The type strain is MobM(T) (=DSM 21213(T)=NBRC 104158(T)).