Acquired contractile ability in human endometrial stromal cells by passive loading of cyclic tensile stretch
Acquired contractile ability in human endometrial stromal cells by passive loading of cyclic tensile stretch
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DOI：
10.1038/s41598-020-65884-3
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发表时间：
2020-06-02
影响因子：
4.6
通讯作者：
Furuakwa, Katsuko S.
中科院分区：
文献类型：
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作者：
Kim, Jeonghyun;Ushida, Takashi;Furuakwa, Katsuko S.
子宫在妊娠期间起着重要而独特的作用，是一个受到机械刺激的动态器官。据报道，不孕症发生时，预防子宫内膜异位症，但其机制仍然不明。在这项研究中，我们发现，机械应变模仿子宫平滑肌层的蠕动运动，使子宫内膜间质细胞获得收缩。为了模拟子宫平滑肌细胞诱导的子宫内膜异位，对人子宫内膜间质细胞施加周期性拉伸。结果表明，应变细胞在催产素存在下在三维胶原凝胶中表现出更大的收缩性，这是由于通过cAMP信号通路上调α-平滑肌肌动蛋白表达。这些体外研究结果强调了子宫内膜间质细胞表型的可塑性，并表明这些细胞在体内获得收缩性的可能性及其对子宫收缩活性的潜在贡献。这种现象可以是组织如何在来自相邻组织的机械刺激下被动地获得新的收缩功能，使其能够支持相邻组织的功能的典型示例。
The uterus plays an important and unique role during pregnancy and is a dynamic organ subjected to mechanical stimuli. It has been reported that infertility occurs when the peristalsis is prevented, although its mechanisms remain unknown. In this study, we found that mechanical strain mimicking the peristaltic motion of the uterine smooth muscle layer enabled the endometrial stromal cells to acquire contractility. In order to mimic the peristalsis induced by uterine smooth muscle cells, cyclic tensile stretch was applied to human endometrial stromal cells. The results showed that the strained cells exerted greater contractility in three-dimensional collagen gels in the presence of oxytocin, due to up-regulated alpha-smooth muscle actin expression via the cAMP signaling pathway. These in vitro findings underscore the plasticity of the endometrial stromal cell phenotype and suggest the possibility of acquired contractility by these cells in vivo and its potential contribution to uterine contractile activity. This phenomenon may be a typical example of how a tissue passively acquires new contractile functions under mechanical stimulation from a neighboring tissue, enabling it to support the adjacent tissue's functions.