Development and polymorphism of microsatellite primers in Ficus pumila L. (Moraceae).
Development and polymorphism of microsatellite primers in Ficus pumila L. (Moraceae).
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DOI：
10.3732/ajb.1000340
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发表时间：
2011-07
影响因子：
3
通讯作者：
Jian Zhang;Kai Jiang;Yi-Su Shi;Min Liu;Xiao‐Yong Chen
中科院分区：
文献类型：
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作者：
Jian Zhang;Kai Jiang;Yi-Su Shi;Min Liu;Xiao‐Yong Chen
研究前提在功能雌雄异株的榕树中开发了微卫星引物，为进一步的群体遗传研究和亲子分析提供多态性标记。方法和结果 在蒺藜中开发了 11 个多态性微卫星位点。这些位点在中国东部的四个蕞尖种群（佛都、象山、仙居和河西）中成功扩增。这些基因座在所有 80 个 F. pumila 个体中都有 3-11 个等位基因。在群体水平上，每个位点的等位基因数量从 1 到 8 不等，观察到的 (H(O)) 和预期 (H(E)) 杂合度分别为 0.000 到 0.900 和 0.000 到 0.830。在四个测试群体中的三个中发现了位点 FP213 和 FP435 之间的连锁不平衡。结论 这些位点表现出高水平的多态性，表明这些引物在群体遗传学研究以及蒺藜亲缘分析中的实用性。
PREMISE OF THE STUDY Microsatellite primers were developed in the functionally dioecious Ficus pumila L. to provide polymorphic markers for further population genetic studies and parentage analysis. METHODS AND RESULTS Eleven polymorphic microsatellite loci were developed in F. pumila. These loci were successfully amplified in four F. pumila populations from eastern China (Fodu, Xiangshan, Xianju, and Hexi). These loci had 3-11 alleles across all 80 F. pumila individuals. At the population level, the number of alleles per locus varied from 1 to 8, and the observed (H(O)) and expected (H(E)) heterozygosities ranged from 0.000 to 0.900 and from 0.000 to 0.830, respectively. Linkage disequilibrium between loci FP213 and FP435 was found in three of the four tested populations. CONCLUSIONS These loci showed high levels of polymorphism, indicating the utility of these primers in population genetic studies as well as parentage analysis of F. pumila.