Bioadsorbable polymer scaffolds for tissue engineering capable of sustained growth factor delivery
Bioadsorbable polymer scaffolds for tissue engineering capable of sustained growth factor delivery
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DOI：
10.1016/s0168-3659(99)00138-8
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发表时间：
2000-02-14
影响因子：
10.8
通讯作者：
Mooney, DJ
中科院分区：
文献类型：
--
作者：
Sheridan, MH;Shea, LD;Mooney, DJ
利用生物可降解聚合物基质上的细胞移植来设计新组织是一种有吸引力的方法，可以治疗患有许多组织和器官丧失或功能障碍的患者。在组织形成过程中，基质必须保持结构的完整性，促进组织的血管化。许多分子（血管生成因子）已经被确定可以促进组织内内皮细胞形成新的血管床，并且这些因子从基质中局部、可控地传递可能会增强工程组织的血管化。我们已经开发出一种气体发泡聚合物加工方法，可以在不使用有机溶剂或高温的情况下，用生物可吸收材料（例如，丙交酯和乙醇酯的共聚物[PLG]）制造三维多孔基质。研究了几种工艺参数（如气体类型、聚合物组成和分子量）对工艺的影响。在制造过程中使用了几种气体（CO2, N-2, He），但只有CO2可以形成高度多孔，结构完整的基体。结晶聚合物（聚丙交酯和聚乙二醇）不能形成多孔基质，而无定形共聚物（50:50,75:25和85:15的丙交酯：乙二醇比例）泡沫生成的基质孔隙率高达95%。基体的力学性能也受PLG组成和分子量的选择的影响。血管生成因子（如血管内皮生长因子）随后在制造过程中加入到基质中，并以受控的方式释放。重要的是，释放的生长因子保留了90%以上的生物活性。总之，一个有前途的系统，血管生成因子从三维，可生物降解的聚合物基质的掺入和输送已经开发出来，制造过程允许在温和的条件下掺入。(C) 2000 Elsevier Science B.V.版权所有
Engineering new tissues utilizing cell transplantation on biodegradable polymer matrices is an attractive approach to treat patients suffering from the loss or dysfunction of a number of tissues and organs. The matrices must maintain structural integrity during the process of tissue formation, and promote the vascularization of the developing tissue. A number of molecules (angiogenic factors) have been identified that promote the formation of new vascular beds from endothelial cells present within tissues, and the localized, controlled delivery of these factors from a matrix may allow an enhanced vascularization of engineered tissues. We have developed a gas foaming polymer processing approach that allows the fabrication of three-dimensional porous matrices from bioabsorbable materials (e.g., copolymers of lactide and glycolide [PLG]) without the use of organic solvents or high temperatures. The effects of several processing parameters (e.g., gas type, polymer composition and molecular weight) on the process were studied. Several gases (CO2, N-2, He) were utilized in the fabrication process, but only CO2 resulted in the formation of highly porous, structurally intact matrices. Crystalline polymers (polylactide and polyglycolide) did not form porous matrices, while amorphous copolymers (50:50, 75:25, and 85:15 ratio of lactide:glycolide) foamed to yield matrices with porosity up to 95%. The mechanical properties of matrices were also regulated by the choice of PLG composition and molecular weight. Angiogenic factors (e.g., vascular endothelial growth factor) were subsequently incorporated into matrices during the fabrication process, and released in a controlled manner. Importantly, the released growth factor retains over 90% of its bioactivity, In summary, a promising system for the incorporation and delivery of angiogenic factors from three-dimensional, biodegradable polymer matrices has been developed, and the fabrication process allows incorporation under mild conditions. (C) 2000 Elsevier Science B.V. All rights reserved.