ISOLATION AND SEQUENCE-ANALYSIS OF A NOVEL HUMAN TYROSINE KINASE GENE
ISOLATION AND SEQUENCE-ANALYSIS OF A NOVEL HUMAN TYROSINE KINASE GENE
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DOI：
10.1128/mcb.9.4.1587
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发表时间：
1989-04-01
影响因子：
5.3
通讯作者：
GROFFEN, J
中科院分区：
文献类型：
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作者：
HAO, QL;HEISTERKAMP, N;GROFFEN, J
使用v-abl探针，我们已经纯化并克隆了一种新的fes/fps同源的人cDNA，我们已指定FER（发音为“公平”）。该3.0内切酶的明显全长cDNA具有2，466个碱基对的开放阅读框架和编码94，000分子量的蛋白质的能力。该cDNA含有与其他癌基因和生长因子受体的高度保守的酪氨酸蛋白激酶结构域同源的区域，但缺乏明确的跨膜区，表明其编码非受体型酪氨酸激酶。推导的氨基酸序列与c-fes/fps相似。我们的数据表明，蛋白质产品的FER，p94 FER，对应于以前报道的细胞磷蛋白，NCP94，检测与v-fps特异性抗肽抗血清。
Using v-abl probes, we have idenified and cloned a novel fes/fps-homologous human cDNA, which we have designated FER (pronounced "fair"). This apparently full-length cDNA of 3.0 kilobases has an open reading frame of 2,466 base pairs and the capacity to encode a protein of 94,000 molecular weight. The cDNA contains regions homologous to the highly conserved tyrosine protein kinase domain of other oncogenes and growth factor receptors but lacks a clear transmembrane region, indicating that it encodes a tyrosine kinase of the nonreceptor type. The deduced amino acid sequences of FER resembles that of c-fes/fps. Our data indicate that the protein product of FER, p94FER, corresponds to a previously reported cellular phosphoprotein, NCP94, detected with a v-fps-specific antipeptide antiserum.