Distinguish oral-source VOCs and control their potential impact on breath biomarkers
Distinguish oral-source VOCs and control their potential impact on breath biomarkers
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区分口腔源 VOC 并控制其对呼吸生物标志物的潜在影响
DOI：
10.1007/s00216-021-03866-8
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发表时间：
2022-01-04
影响因子：
4.3
通讯作者：
Chu, Yannan
中科院分区：
文献类型：
--
作者：
Ge, Dianlong;Zhou, Jijuan;Chu, Yannan
采用玻璃瓶取样-固相微萃取-气相色谱-质谱联用（SPME-GC-MS）技术，研究了41例正常人和50例食管癌患者在漱口前后呼吸中挥发性有机物（VOCs）的变化特征。采用非目标策略，对143个VOC色谱峰进行统计分析。基于正交偏最小二乘判别分析（OPLS-DA），根据投影变量重要度（VIP > 1.5）、Wilcoxon符号秩检验（P < 0.05）和折叠变化（FC > 2.0）的联合标准，获得各呼吸试验组含漱后VOC的变化情况。漱口时，健康人呼吸中的吲哚、苯酚、1-丙醇和对甲酚含量降低；食管癌患者呼吸中下降的VOCs为吲哚、苯酚、二甲二硫和对甲酚。特别是，这些物质以前被报道为食道癌、胃癌、甲状腺癌、乳腺癌、口腔癌和肺癌等一些疾病以及某些非癌症疾病的呼吸生物标志物。目前的工作表明，呼气挥发性有机化合物涉及口腔的主要来源，在呼吸生物标志物研究中，应考虑来自口腔挥发物的潜在影响。鉴于目前的结果，还提出漱口水预处理可以消除口腔voc可能的干扰，这可能有利于呼吸生物标志物的研究。
By means of glass bottle sampling followed by solid-phase microextraction gas chromatography-mass spectrometry (SPME-GC-MS) technique, the change characteristics of volatile organic compounds (VOCs) in breaths, between before gargling and after gargling, were investigated, respectively, in 41 healthy subjects and 50 esophageal cancer patients. Using an untargeted strategy, 143 VOC chromatographic peaks were enrolled in the statistical analysis. Based on the orthogonal partial least squares discriminant analysis (OPLS-DA), the VOC variations after gargling for each breath test group were obtained according to the combined criteria of variable importance in projection (VIP > 1.5), Wilcoxon signed-rank test (P < 0.05), and fold change (FC > 2.0). When gargled, the levels of indole, phenol, 1-propanol, and p-cresol in the breath of healthy people decreased; meanwhile, for esophageal cancer patients, the declined VOCs in breath were indole, phenol, dimethyl disulfide, and p-cresol. Particularly, these substances were previously reported as breath biomarkers in some diseases such as esophageal, gastric, thyroid, breast, oral, and lung cancers, as well as certain non-cancer disorders. The present work indicates that expiratory VOCs involve the prominent oral cavity source, and in the breath biomarkers study, the potential impact that originates from oral volatiles should be considered. In view of the present results, it is also proposed that gargle pretreatment could eliminate possible interference from the oral cavity VOCs that might benefit breath biomarker investigation.