Arginine kinases from the precious corals Corallium rubrum and Paracorallium japonicum: presence of two distinct arginine kinase gene lineages in cnidarians.
Arginine kinases from the precious corals Corallium rubrum and Paracorallium japonicum: presence of two distinct arginine kinase gene lineages in cnidarians.
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来自珍贵珊瑚 Corallium rubrum 和 Paracorallium japonicum 的精氨酸激酶：在刺胞动物中存在两种不同的精氨酸激酶基因谱系。
DOI：
10.1007/s10930-017-9745-7
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
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作者：
Matsuo;T.;Yano;D.;Uda;K.;Iwasaki;N.;and Suzuki;T.
从转录组序列数据中组装了珍贵的珊瑚红色珊瑚精氨酸激酶(AK)的cDNA序列，推导出的364个氨基酸序列保留了AK的结构特征。根据氨基酸序列，DNA编码C。采用重叠延伸聚合酶链式反应合成RubrumAK，制备重组酶。测定了C的下列动力学参数。红细胞酶：KaArg(0.10 mm)，KiaArg(0.79 mm)，KaATP(0.23 mm)，KiaATP(2.16 mm)，KCAT(74.3.S−1)。这些参数与其他AKs的动力学参数是相当的。然而，系统发育分析表明，C。RubrumAK序列与其他已知的具有不寻常的双域结构的Cnnidaria AK序列具有明显的起源。使用根据OFC序列设计的低聚物。成功地扩增了另一种珊瑚副冠藻基因组DNA的编码区rubrumAK。虽然两个AK的核苷酸序列在编码区的14个位置不同，但都涉及同义替换，得到相同的氨基酸序列。塞普。与其他日本AK基因相比，日本AK基因在一个独特的位置含有一个内含子。结合系统发育分析的观察结果，外显子/内含子组织的比较支持两种截然不同的AK基因谱系的观点。Fc编码区之间的核苷酸序列的差异。红色和P。日本血吸虫AKs为1.28%，是线粒体DNA(0.54%)的两倍，与线虫线粒体基因组进化慢于核基因组的普遍观察结果一致。
The cDNA sequence of arginine kinase (AK) from the precious coralCorallium rubrumwas assembled from transcriptome sequence data, and the deduced amino acid sequence of 364 residues was shown to conserve the structural features characteristic of AK. Based on the amino acid sequence, the DNA codingC. rubrumAK was synthesized by overlap extension PCR to prepare the recombinant enzyme. The following kinetic parameters were determined for theC. rubrumenzyme:KaArg(0.10 mM),KiaArg(0.79 mM),KaATP(0.23 mM),KiaATP(2.16 mM), andkcat(74.3 s−1). These are comparable with the kinetic parameters of other AKs. However, phylogenetic analysis suggested that theC. rubrumAK sequence has a distinct origin from that of other known cnidarian AKs with unusual two-domain structure. Using oligomers designed from the sequence ofC. rubrumAK, the coding region of genomic DNA of another coralParacorallium japonicumAK was successfully amplified. Although the nucleotide sequences differed between the two AKs at 14 positions in the coding region, all involved synonymous substitutions, giving the identical amino acid sequence. TheP. japonicumAK gene contained one intron at a unique position compared with other cnidarian AK genes. Together with the observations from phylogenetic analysis, the comparison of exon/intron organization supports the idea that two distinct AK gene lineages are present in cnidarians. The difference in the nucleotide sequence between the coding regions ofC. rubrumandP. japonicumAKs was 1.28%, which is twice that (0.54%) of mitochondrial DNA, is consistent with the general observation that the mitochondrial genome evolves slower than the nuclear one in cnidarians.