Primary influenza A virus infection induces cross-protective immunity against a lethal infection with a heterosubtypic virus strain in mice
Primary influenza A virus infection induces cross-protective immunity against a lethal infection with a heterosubtypic virus strain in mice
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DOI：
10.1016/j.vaccine.2006.08.036
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发表时间：
2007-01-08
期刊：
影响因子：
5.5
通讯作者：
Rimmelzwaan, G. F.
中科院分区：
文献类型：
--
作者：
Kreijtz, J. H. C. M.;Bodewes, R.;Rimmelzwaan, G. F.
为了评估初次感染另一亚型病毒株对致命流感病毒感染的保护水平，C57BL/6小鼠感染了亚致死性流感病毒X-31(H3N2)，随后用致命毒株A/PR/8/34(H1N1)攻击。将挑战感染的结果与在挑战感染之前没有经历过流感病毒X-31感染的小鼠进行比较。X-31有经验的小鼠以加速的方式清除了流感病毒A/PR/8/34的感染，临床症状较少，肺部病变减少，与幼稚小鼠相比，这些小鼠的存活率提高了。通过检测流感病毒X-31和A/PR/8/34所共有的H-2D(B)限制性NP366-374表位的细胞毒性T淋巴细胞(CTL)反应，证明了在X-31经历过的小鼠中，挑战感染流感病毒A/PR/8/34的改善结果与记忆病毒特异性CD8(+)细胞毒性T淋巴细胞(CTL)反应有关。因此，在没有针对血凝素和神经氨酸酶的病毒中和抗体的情况下，以前接触甲型流感病毒对感染提供了部分保护。根据当前的大流行威胁和旨在诱导病毒特异性CTL的疫苗的开发，讨论了这些观察结果的影响。(C)2006爱思唯尔有限公司。保留所有权利。
In order to assess the level of protection against a lethal influenza virus infection provided by a primary infection with a virus strain of another subtype, C57BL/6 mice were infected with the sublethal influenza virus X-31 (H3N2) and subsequently challenged with the lethal strain A/PR/8/34 (H1N1). The outcome of the challenge infection was compared with that in mice that did not experience an infection with influenza virus X-31 prior to the challenge infection. The X-31 experienced mice cleared the infection with influenza virus A/PR/8/34 in ail accelerated fashion, displayed less clinical signs and a reduction of lesions in the lungs resulting in improved survival rates of these mice compared to the naive mice. The improved outcome of the challenge infection with influenza virus A/PR/8/34 in the X-31 experienced mice correlated with priming for anamnestic virus-specific CD8(+) cytotoxic T lymphocyte (CTL) responses as was demonstrated by the detection of CTL specific for the H-2D(b) restricted NP366-374 epitope that was shared by the influenza viruses X-31 and A/PR/8/34. Thus previous exposure to influenza A viruses affords partial protection against infection in the absence of virus-neutralizing antibodies specific for the hemagglutinin and the neuraminidase. The implications of these observations are discussed in the light of the current pandemic threat and development of vaccines that aim at the induction of virus-specific CTL. (c) 2006 Elsevier Ltd. All rights reserved.