Ultrastructural double-labeling demonstrates synaptic contacts between dopaminergic terminals and substance P-containing striatal neurons in pigeons.
Ultrastructural double-labeling demonstrates synaptic contacts between dopaminergic terminals and substance P-containing striatal neurons in pigeons.
复制标题
超微结构双标记显示鸽子多巴胺能末端和含有 P 物质的纹状体神经元之间的突触接触。
DOI：
10.1016/0006-8993(92)90490-z
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发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Reiner,A
中科院分区：
文献类型：
--
作者：
Karle,EJ;Anderson,KD;Reiner,A
Immunohistochemical studies in rats have demonstrated dopaminergic input onto medium spiny neurons of the striatum. Medium spiny neurons, however, are known to consist of two major neuropeptide-specific types, those containing substance P (SP) and those containing enkephalin. Although both of these types have been shown to receive dopaminergic input onto their perikarya and proximal dendrites, the extent to which both types also receive direct dopaminergic input onto distal dendritic shafts or onto dendritic spines is uncertain. In the present study, we used EM immunohistochemical double-label techniques to examine the synaptic organization of dopaminergic input onto SP+striatal neurons. We examined the striatum of pigeons, in whom SP+striatal neurons, including their dendritic shafts and spines, can be readily labeled. Antibodies against tyrosine hydroxylase (TH) were used to identify dopaminergic terminals, which were labeled using silver-intensified immunogold. The SP+neurons were labeled immunohistochemically using diaminobenzidine. We found that dopaminergic terminals make appositions and form symmetric synapses with the perikarya, dendritic shafts and dendritic spines of SP+neurons. Thus, nigral dopaminergic neurons provide a monosynaptic input onto SP+striatal neurons in a manner similar to that described for dopaminergic input onto striatal medium spiny neurons in general.
