The 5' flanking region of human epsilon-globin gene.
The 5' flanking region of human epsilon-globin gene.
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人类ε珠蛋白基因的 5 侧翼区域。
DOI：
10.1093/nar/8.19.4393
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发表时间：
1980
影响因子：
14.9
通讯作者：
N. Proudfoot
中科院分区：
文献类型：
--
作者：
F. Baralle;C. Shoulders;S. Goodbourn;A. Jeffreys;N. Proudfoot
对epsilon-globin基因上游2.0 kb区域进行了结构分析。详细绘制了该基因周围的基因组 DNA 图谱，以确保克隆的 DNA 能够代表实际的染色体排列。此外，还开发了一种新技术，可将 1.5 kb 处的重复 DNA 序列精确定位到基因 5' 侧。然后确定 2.0 kb 5' 侧翼区域的完整核苷酸序列并与该基因重叠。该序列包括与所谓的 AluI 重复家族同源的重复 DNA 序列。重复序列的 5' 和 3' 侧均存在 50 个核苷酸长的异常序列，其中 A + T 代表约 90% 的碱基。
The structural analysis of the 2.0 kb region upstream from the epsilon-globin gene has been carried out. A genomic DNA map around the gene was worked out in some detail to ensure that the cloned DNA was representative of the actual chromosomal arrangement. Furthermore, a new technique was developed to precisely map a reiterated DNA sequence present 1.5 kb to the 5' side of the gene. The complete nucleotide sequence of the 2.0 kb 5' flanking region was then determined and overlapped with the gene. The sequence included the reiterated DNA sequence which is homologous to the so-called AluI family of repeats. Unusual stretches of sequence 50 nucleotides long, where A + T represent about 90% of the bases, are present at both the 5' and 3' sides of the repeat.