An increase in cAMP concentration in mouse hippocampal slices exposed to low-frequency and pulsed magnetic fields.
An increase in cAMP concentration in mouse hippocampal slices exposed to low-frequency and pulsed magnetic fields.
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DOI：
10.1016/j.neulet.2004.05.006
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发表时间：
2004-08-05
影响因子：
2.5
通讯作者：
Wieraszko, A
中科院分区：
文献类型：
--
作者：
Hogan, MV;Wieraszko, A
我们以前的研究表明，磁场放大了从小鼠海马片记录到的诱发电位。为了探索这种效应的机制，我们评估了暴露在低频和脉冲磁场中的脑片中cAMP的浓度。15mT的低频磁场以0.16赫兹作用30min，使cAMP浓度增加近3倍。在关闭磁场后的第一个小时内，cAMP浓度继续上升，几乎增加了四倍，然后在第二个小时结束时恢复到对照水平。静磁场和频率为0.5赫兹的磁场对cAMP浓度均无影响。CAMP水平的增加依赖于磁场的强度，并需要细胞外钙的存在。施加不同强度的脉冲磁场(9-15mT)，周期为5-20分钟，cAMP浓度增加一倍。这些结果支持我们以前的电生理观察，并为它们的解释提供了生化关联。(C)2004爱思唯尔爱尔兰有限公司。保留所有权利。
Our previous studies revealed that magnetic fields amplified evoked potentials recorded from mouse hippocampal slices. In search for the mechanism of this effect, we evaluated the concentration of cAMP in slices exposed to low-frequency and pulsed magnetic fields. Low-frequency magnetic fields of 15 mT applied at 0.16 Hz for 30 min enhanced the concentration of cAMP almost three-fold. The concentration of cAMP continued to rise through the first hour after turning magnetic fields off, reaching almost a four-fold increase, and then returned to control levels at the end of the second hour. Neither static magnetic fields nor magnetic fields applied with the frequency of 0.5 Hz had any effect on cAMP concentration. The increase in cAMP levels was dependent on the strength of the magnetic field and required the presence of extracellular calcium. A pulsed magnetic field applied with variable intensity (9-15 mT) and in cycles lasting from 5 to 20 min doubled the cAMP concentration. These results support our previous electrophysiological observations and provide biochemical correlates for their interpretation. (C) 2004 Elsevier Ireland Ltd. All rights reserved.