PHAGOCYTOSIS IN ACANTHAMOEBA .1. A MANNOSE RECEPTOR IS RESPONSIBLE FOR THE BINDING AND PHAGOCYTOSIS OF YEAST
PHAGOCYTOSIS IN ACANTHAMOEBA .1. A MANNOSE RECEPTOR IS RESPONSIBLE FOR THE BINDING AND PHAGOCYTOSIS OF YEAST
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DOI：
10.1002/jcp.1041450317
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发表时间：
1990-12-01
影响因子：
5.6
通讯作者：
DAWIDOWICZ, EA
中科院分区：
文献类型：
--
作者：
ALLEN, PG;DAWIDOWICZ, EA
为了在分子水平上了解这一过程，我们研究了卡氏阿米巴的吞噬作用的初始事件，并确定了这种生物体的吞噬作用是由一种受体介导的，该受体识别被吞噬颗粒中富含甘露糖的元素。 我们证明，在酵母颗粒的结合和内化可以抑制糖D（+）-甘露糖和D（-）-果糖在立体特异性，浓度依赖性的方式。 这种抑制是特异性的;这些糖不会抑制这种生物体对乳胶珠的摄取。 使用mannoslyated neoglycoproteins，这是更有效的抑制剂的颗粒结合相比，游离糖，我们证明了存在的受体的阿米巴细胞表面上的酵母菌的结合作为吞噬作用的初始事件是必要的。 阿米巴甘露糖受体似乎也能够介导将可溶性富含甘露糖的分子递送至降解隔室如溶酶体。 对这种受体的了解将使我们更好地理解吞噬作用的分子事件。
We have examined the initial events in phagocytosis by Acanthamoebe castellanii in order to understand this process at the molecular level and have determined that phagocytosis in this organism is mediated by a receptor which recognizes mannose-rich elements in the particle to be phagocytosed. We demonstrate that the binding and internalization at yeast particles can be inhibited by the sugars D(+)-mannose and D(-)-fructose in a stereospecific, concentration-dependent manner. This inhibition is specific; these sugars did not inhibit the uptake of latex beads by this organism. Using mannoslyated neoglycoproteins, which are much more potent inhibitors of particle binding as compared with the free sugar, we demonstrate the presence of a receptor on the amoeba cell surface which is necessary for the binding of yeast as the initial event of phagocytosis. The Acanthamoeba mannose receptor also appears to be able to mediate the delivery a soluble mannose-rich molecules to a degradative compartment such as the lysosome. Knowledge of this receptor will allow a better understanding of the molecular events of phagocytosis.