Influence of probiotic vaginal lactobacilli on in vitro adhesion of urogenital pathogens to vaginal epithelial cells
Influence of probiotic vaginal lactobacilli on in vitro adhesion of urogenital pathogens to vaginal epithelial cells
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DOI：
10.1111/j.1472-765x.2006.01934.x
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发表时间：
2006-08-01
影响因子：
2.4
通讯作者：
Nader-Macias, M. E.
中科院分区：
文献类型：
--
作者：
Zarate, G.;Nader-Macias, M. E.
目的：乳酸杆菌是阴道微生物群的主要微生物，通过防止病原菌的定植在维持健康的泌尿生殖道中发挥主要作用。本研究的目的是评估四种阴道乳杆菌属菌株（先前根据其益生菌特征选择）在体外阻断三种人类泌尿生殖道病原体对阴道上皮细胞（VEC）的粘附的能力。方法和结果：进行三种类型的测定以确定乳杆菌对泌尿生殖道病原体对VEC的粘附的抑制作用：通过排除（乳酸杆菌和VEC，随后是病原体）、竞争（乳酸杆菌、VEC和病原体一起）和置换（病原体和VEC，随后是乳酸杆菌的加入）进行的阻断。革兰氏染色后，通过显微镜（x1000）定量细菌粘附至VEC。所有的菌株都能够通过排斥和竞争抑制金黄色葡萄球菌对VEC的粘附，但没有一种菌株能够通过所测定的两种机制降低大肠杆菌的粘附。只有嗜酸乳杆菌CRL 1259和副干酪乳杆菌CRL 1289分别通过排斥和竞争作用抑制B族链球菌（GBS）与VEC的粘附。研究的意义和影响：结果支持这些乳杆菌属菌株作为阴道中抗感染剂的益生菌潜力，并鼓励进一步研究其预防和管理女性泌尿生殖道感染的能力。
Aims: Lactobacilli, the predominant micro-organisms of the vaginal microbiota, play a major role in the maintenance of a healthy urogenital tract by preventing the colonization of pathogenic bacteria. The aim of the present study was to assess the ability of four vaginal Lactobacillus strains, previously selected for their probiotic features, to block in vitro the adherence of three human urogenital pathogens to vaginal epithelial cells (VEC).Methods and Results: Three types of assays were performed in order to determine the inhibitory effect of lactobacilli on adhesion of urogenital pathogens to VEC: blockage by exclusion (lactobacilli and VEC followed by pathogens), competition (lactobacilli, VEC and pathogens together) and displacement (pathogens and VEC followed by the addition of lactobacilli). Bacterial adhesion to VEC was quantified by microscopy (x1000) after Gram's stain. All the strains were able to inhibit by exclusion and competition the adhesion of Staphylococcus aureus to VEC but none was able to decrease the attachment of Escherichia coli by neither of the mechanisms assayed. Only Lactobacillus acidophillus CRL 1259 and Lactobacillus paracasei CRL 1289 inhibited the attachment of Group B streptococci (GBS) to VEC by exclusion and competition respectively.Conclusions: Lactobacillus of vaginal origin were able to inhibit the attachment of genitouropathogenic Staph. aureus and GBS to the vaginal epithelium.Significance and Impact of the Study: The results support the probiotic potential of these Lactobacillus strains as anti-infective agents in the vagina and encourage further studies about their capacity to prevent and manage urogenital tract infections in females.