Prostaglandin Receptor EP1 and EP2 Site in Guinea Pig Bladder Urothelium and Lamina Propria
Prostaglandin Receptor EP1 and EP2 Site in Guinea Pig Bladder Urothelium and Lamina Propria
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DOI：
10.1016/j.juro.2009.11.004
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发表时间：
2010-03-01
影响因子：
6.6
通讯作者：
Gillespie, James I.
中科院分区：
文献类型：
--
作者：
Rahnama'i, Mohammad Sajjad;van Koeveringe, Gommert A.;Gillespie, James I.
用途：尿路上皮有两个主要功能。它是尿液的屏障，具有感觉作用。作为对拉伸的反应，尿路上皮释放各种调节传入神经活动的物质。材料与方法：对7只豚鼠膀胱进行前列腺素1型和2型受体染色，并对vimentin和环氧合酶i进行染色。结果：尿路上皮细胞和尿路上皮下可见前列腺素1型受体染色。尿路上皮染色在所有的尿路上皮细胞层，包括尿路上皮下细胞中均可见，染色经常是间断的和微弱的。相反，在尿路上皮和上皮下细胞中可见强烈的前列腺素受体2型染色。间质细胞和伞状细胞中不存在环加氧酶I，在基底细胞层中含量最高。结论：间质细胞表达前列腺素受体1型和2型，表明它们对前列腺素有应答。伞状细胞不表达环加氧酶I。环加氧酶I存在于基底尿路上皮细胞中，使其成为前列腺素合成的可能位点。因此，尿路上皮产生的前列腺素可能靶向尿路上皮和尿路上皮下的前列腺素受体1型和2型。因此，前列腺素被假设在膀胱壁的信号调节中起作用。
Purpose: Urothelium has 2 main functions. It is a barrier to urine and has a sensory role. In response to stretch urothelium releases various substances that modulate afferent nerve activity. Recent data on the localization of cyclooxygenase type 1, the enzyme responsible for prostaglandin production, suggests that prostaglandin may have complex local action..Materials and Methods: The bladders of 7 guinea pigs were stained for prostaglandin receptors type 1 and 2, and costained for vimentin and cyclooxygenase I.Results: Prostaglandin receptor type 1 staining was seen in urothelial cells and in the suburothelium. Urothelial staining, which was often punctuate and weak, was detected in all urothelial cell layers, including suburothelial cells. In contrast, strong prostaglandin receptor type 2 staining was seen in the urothelium and in suburothelial cells. Cyclooxygenase I was absent in interstitial cells and umbrella cells with the highest concentration in the basal cell layer.Conclusions: Interstitial cells express prostaglandin receptor types 1 and 2, indicating that they can respond to prostaglandin. Umbrella cells do not express cyclooxygenase I. Cyclooxygenase I was present in basal urothelial cells, making them a possible site of prostaglandin synthesis. Thus, prostaglandin produced by urothelium may target prostaglandin receptor types 1 and 2 in the urothelium and suburothelium. Therefore prostaglandin is hypothesized to have a role in signal regulation in the bladder wall.